A single step purification for recombinant proteins Characterization of a microtubule associated protein (MAP 2) fragment which associates with the type II cAMP‐dependent protein kinase

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

The purification of eukaryotic polypeptides synthesized in Escherichia coli

1986 • 838 citations

Interaction of the regulatory subunit (RII) of cAMP-dependent protein kinase with RII-anchoring proteins occurs through an amphipathic helix binding motif

1991 • 437 citations

[29] Synthesis and sequence-specific proteolysis of hybrid proteins produced in Escherichia coli

1987 • 392 citations

Generation of β-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli

1984 • 374 citations

[28] Assay of coagulation proteases using peptide chromogenic and fluorogenic substrates

1981 • 310 citations

Identification of the calmodulin-binding domain of skeletal muscle myosin light chain kinase.

1985 • 302 citations

A novel strategy for production of a highly expressed recombinant protein in an active form

1991 • 218 citations

The minimum substrate of cyclic AMP-stimulated protein kinase, as studied by synthetic peptides representing the phosphorylatable site of pyruvate kinase (type L) of rat liver

1976 • 214 citations

Calmodulin-binding proteins of the microfilaments present in isolated brush borders and microvilli of intestinal epithelial cells.

1980 • 210 citations

Synthetic hexapeptide substrates and inhibitors of 3':5'-cyclic AMP-dependent protein kinase.

1976 • 206 citations

Type II regulatory subunit dimerization determines the subcellular localization of the cAMP-dependent protein kinase.

1990 • 183 citations

Identification of an inhibitory region of the heat-stable protein inhibitor of the cAMP-dependent protein kinase.

1985 • 174 citations

A Polypeptide Fusion Designed for the Purification of Recombinant Proteins

1984 • 146 citations

Gene fusion vectors based on the gene for staphylococcal protein A

1983 • 137 citations

The RII subunit of camp-dependent protein kinase binds to a common amino-terminal domain in microtubule-associated proteins 2A, 2B, and 2C

1989 • 130 citations

Localization and characterization of the binding site for the regulatory subunit of type II cAMP-dependent protein kinase on MAP2

1989 • 128 citations

Amino acid sequence of rabbit skeletal muscle myosin light chain kinase

1986 • 96 citations

Use of gene fusions and protein-protein interaction in the isolation of a biologically active regulatory protein: the replication initiator protein of plasmid R6K.

1983 • 84 citations

Production of specific antibodies against protein A fusion proteins.

1986 • 77 citations

Open reading frame cloning: identification, cloning, and expression of open reading frame DNA.

1982 • 70 citations

Construction of an excretion vector and extracellular production of human growth hormone from Escherichia coli

1987 • 68 citations

Isolation of the cDNA encoding rat skeletal muscle myosin light chain kinase. Sequence and tissue distribution.

1988 • 56 citations