Selection of human antibody fragments on the basis of stabilization of the variable domain in the presence of target antigens

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

Making Antibodies by Phage Display Technology

1994 • 1,516 citations

Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides 1 1Edited by I. A. Wilson

2000 • 722 citations

Crystallographic Evidence for Preformed Dimers of Erythropoietin Receptor Before Ligand Activation

1999 • 597 citations

Functional Mimicry of a Protein Hormone by a Peptide Agonist: The EPO Receptor Complex at 2.8 Å

1996 • 589 citations

Interactions of protein antigens with antibodies.

1996 • 543 citations

Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system

1997 • 485 citations

Recombining germline-derived CDR sequences for creating diverse single-framework antibody libraries

2000 • 330 citations

Direct Random Mutagenesis of Gene-Sized DNA Fragments Using Polymerase Chain Reaction

1995 • 272 citations

Phage display in pharmaceutical biotechnology

2000 • 254 citations

Human erythropoietin receptor: cloning, expression, and biologic characterization

1990 • 242 citations

Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent—application to a human single-chain Fv fragment

1998 • 195 citations

X-Ray Crystallography of Antibodies

1996 • 169 citations

Open sandwich ELISA: A novel immunoassay based on the interchain interaction of antibody variable region

1996 • 154 citations

High copy display of large proteins on phage for functional selections 1 1Edited by P. E. Wright

2000 • 122 citations

Beyond binding: using phage display to select for structure, folding and enzymatic activity in proteins

1999 • 112 citations

[29] Design and use of phage display libraries for the selection of antibodies and enzymes

2000 • 105 citations

The basics of binding: mechanisms of antigen recognition and mimicry by antibodies

1993 • 83 citations

Inclusion of an upstream transcriptional terminator in phage display vectors abolishes background expression of toxic fusions with coat protein g3p

1996 • 73 citations

In vitro abzyme evolution to optimize antibody recognition for catalysis

2001 • 64 citations

Improving the display of proteins on filamentous phage

2001 • 45 citations

A Stable Phage-Display System Using a Phagemid Vector: Phage Display of Hen Egg-White Lysozyme (HEL),Escherichia coliAlkaline, Phosphatase, and Anti-HEL Monoclonal Antibody, HyHEL10

1996 • 33 citations

Thermodynamic Consequences of Grafting Enhanced Affinity toward the Mutated Antigen onto an Antibody

2000 • 21 citations

A phage display vector with improved stability, applicability and ease of manipulation

1995 • 18 citations

Myosin-Reactive Autoantibodies in Rheumatic Carditis and Normal Fetus

1998 • 17 citations

Isolation and Characterization of Monoclonal Antibodies That Inhibit Hepatitis C Virus NS3 Protease

2000 • 16 citations

An improved phage display vector for antibody repertoire cloning by construction of combinatorial libraries

1997 • 10 citations

Phage library panning against cytosolic fraction of cells using quantitative dot blotting assay: application of selected VH to histochemistry

2002 • 8 citations

Panning of a Phage VH Library Using Nitrocellulose Membranes: Application to Selection of a Human VH Library

2001 • 8 citations