Development of a Bicistronic Vector Driven by the Human Polypeptide Chain Elongation Factor 1α Promoter for Creation of Stable Mammalian Cell Lines That Express Very High Levels of Recombinant Proteins

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

pEF-BOS, a powerful mammalian expression vector

1990 • 1,641 citations

Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5' sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system.

1989 • 401 citations

The novel mechanism of initiation of picornavirus RNA translation

1990 • 368 citations

Bicistronic Vector for the Creation of Stable Mammalian Cell Lines that Predisposes All Antibiotic-Resistant Cells to Express Recombinant Protein

1996 • 288 citations

Use of the human elongation factor 1α promoter as a versatile and efficient expression system

1990 • 273 citations

Cap-Independent Translation of Picornavirus RN As: Structure and Function of the Internal Ribosomal Entry Site

1990 • 205 citations

Efficient transformation of mammalian cells with constructs containing a puromycin-resistance marker

1988 • 146 citations

The prokaryotic neomycin-resistance-encoding gene acts as a transcriptional silencer in eukaryotic cells

1991 • 139 citations

The Role of Eucaryotic Elongation Factor Tu in Protein Synthesis

1980 • 137 citations

IRES Bicistronic Expression Vectors for Efficient Creation of Stable Mammalian Cell Lines

1996 • 101 citations

Location of the internal ribosome entry site in the 5' non-coding region of the immunoglobulin heavy-chain binding protein (BiP) mRNA: evidence for specific RNA-protein interactions

1997 • 96 citations

Retroviral Coexpression of a Multidrug Resistance Gene (MDR1) and Humanα-Galactosidase A for Gene Therapy of Fabry Disease

1995 • 83 citations

Characterization of the regulatory elements in the promoter of the human elongation factor-1 alpha gene.

1994 • 78 citations

Pyruvate carboxylase

1998 • 63 citations

Amerindian Pyruvate Carboxylase Deficiency Is Associated with Two Distinct Missense Mutations

1998 • 57 citations

[33] pac Gene as efficient dominant marker and reporter gene in mammalian cells

1992 • 56 citations

The Rat Pyruvate Carboxylase Gene Structure

1997 • 55 citations

Equal transcription of wild-type and mutant p53 using bicistronic vectors results in the wild-type phenotype.

1994 • 48 citations

Electron microscopic localization of pyruvate carboxylase in rat liver and Saccharomyces cerevisiae by immunogold procedures

1991 • 42 citations

Lack of a role for MRP1 in platinum drug resistance in human ovarian cancer cell lines

1998 • 39 citations

Molecular Characterization of Pyruvate Carboxylase Deficiency in Two Consanguineous Families

1998 • 37 citations

Resistance to foot-and-mouth disease virus mediated by trans-acting cellular products

1989 • 26 citations

Bicistronic and Two-Gene Retroviral Vectors for UsingMDR1 as a Selectable Marker and a Therapeutic Gene

1996 • 25 citations