A computer-based protocol for semiautomated assignments and 3D structure determination of proteins

NMR of Proteins and Nucleic Acids

2006 • 5,989 citations

Pure absorption gradient enhanced heteronuclear single quantum correlation spectroscopy with improved sensitivity

1992 • 2,231 citations

The chemical shift index: a fast and simple method for the assignment of protein secondary structure through NMR spectroscopy

1992 • 2,028 citations

A receptor for the immuno-suppressant FK506 is a cis–trans peptidyl-prolyl isomerase

1989 • 1,376 citations

Pseudo-structures for the 20 common amino acids for use in studies of protein conformations by measurements of intramolecular proton-proton distance constraints with nuclear magnetic resonance

1983 • 1,012 citations

Correlating backbone amide and side chain resonances in larger proteins by multiple relayed triple resonance NMR

1992 • 1,012 citations

A cytosolic binding protein for the immunosuppressant FK506 has peptidyl-prolyl isomerase activity but is distinct from cyclophilin

1989 • 1,010 citations

Overcoming the overlap problem in the assignment of proton NMR spectra of larger proteins by use of three-dimensional heteronuclear proton-nitrogen-15 Hartmann-Hahn-multiple quantum coherence and nuclear Overhauser-multiple quantum coherence spectroscopy: application to interleukin 1.beta.

1989 • 840 citations

A novel approach for sequential assignment of proton, carbon-13, and nitrogen-15 spectra of larger proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin

1990 • 836 citations

Determination of three‐dimensional structures of proteins from interproton distance data by hybrid distance geometry‐dynamical simulated annealing calculations

1988 • 775 citations

A common sense approach to peak picking in two-, three-, and four-dimensional spectra using automatic computer analysis of contour diagrams

1991 • 682 citations

Sequential resonance assignments as a basis for determination of spatial protein structures by high resolution proton nuclear magnetic resonance

1982 • 568 citations

Stereospecific nuclear magnetic resonance assignments of the methyl groups of valine and leucine in the DNA-binding domain of the 434 repressor by biosynthetically directed fractional carbon-13 labeling

1989 • 567 citations

Heteronuclear three-dimensional nmr spectroscopy. A strategy for the simplification of homonuclear two-dimensional NMR spectra

1988 • 382 citations

Experiments for recording pure-absorption heteronuclear correlation spectra using pulsed field gradients

1992 • 376 citations

An evaluation of the combined use of nuclear magnetic resonance and distance geometry for the determination of protein conformations in solution

1985 • 373 citations

Determination of Three-Dimensional Structures of Proteins and Nucleic Acids in Solution by Nuclear Magnetic Resonance Spectroscop

1989 • 342 citations

Four-Dimensional Heteronuclear Triple-Resonance NMR Spectroscopy of Interleukin-1β in Solution

1990 • 308 citations

Optimized recording of heteronuclear multidimensional NMR spectra using pulsed field gradients

1992 • 264 citations

Efficient analysis of protein 2D NMR spectra using the software packageEASY

1991 • 240 citations

Sequential resonance assignments in protein 1H nuclear magnetic resonance spectra

1982 • 195 citations

TWO-DIMENSIONAL NMR AND PROTEIN STRUCTURE

1989 • 183 citations

ANSIG: A program for the assignment of protein 1H 2D NMR spectra by interactive computer graphics

1989 • 178 citations

Four-dimensional carbon-13/carbon-13-edited nuclear Overhauser enhancement spectroscopy of a protein in solution: application to interleukin 1.beta.

1991 • 162 citations

Stereospecific assignment of the methyl ¹H NMR lines of valine and leucine in polypeptides by nonrandom ¹³C labelling

1989 • 149 citations

Side chain and backbone assignments in isotopically labeled proteins from two heteronuclear triple resonance experiments

1992 • 130 citations

Distribution of chemical shifts in 1H nuclear magnetic resonance spectra of proteins

1988 • 109 citations

FR-900520 and FR-900523, novel immunosuppressants isolated from a Streptomyces. II. Fermentation, isolation and physico-chemical and biological characteristics.

1988 • 107 citations

[10] Molecular dynamics simulation techniques for determination of molecular structures from nuclear magnetic resonance data

1989 • 100 citations

Sequential individual resonance assignments in the ¹H‐NMR spectra of polypeptides and proteins

1983 • 99 citations

Toward automated assignment of nuclear magnetic resonance spectra: pattern recognition in two-dimensional correlation spectra

1985 • 92 citations

Four-dimensional [13C,1H,13C,1H] HMQC-NOE-HMQC NMR spectroscopy: resolving tertiary nuclear Overhauser effect distance constraints in the spectra of larger proteins

1991 • 89 citations

A 4D HCCH-TOCSY experiment for assigning the side chain1H and13C resonances of proteins

1992 • 84 citations

Three-dimensional structure of the FK506 binding protein/ascomycin complex in solution by heteronuclear three- and four-dimensional NMR

1993 • 76 citations

Ascomycin, an antifungal antibiotic.

1962 • 65 citations

Computer-aided sequential assignment of protein 1H NMR spectra

1988 • 56 citations

NMR studies of an FK-506 analog [U-13C]ascomycin, bound to FKBP: conformation and regions of ascomycin involved in binding

1991 • 55 citations

A program for semi-automatic sequential resonance assignments in protein 1H nuclear magnetic resonance spectra

1988 • 55 citations

Uniform carbon-13 isotope labeling of proteins with sodium acetate for NMR studies: application to human carbonic anhydrase II

1991 • 54 citations

Computer aided evaluation of two-dimensional NMR spectra of proteins

1984 • 53 citations

Programs for computer-assisted sequential assignment of proteins

1989 • 50 citations

Automated recognition and assessment of cross peaks in two-dimensional NMR spectra of macromolecules

1987 • 50 citations

z-Filtered double-quantum NMR spectra and automated analysis by pattern recognition

1987 • 47 citations

Effective combination of gradients and crafted RF pulses for water suppression in biological samples

1992 • 43 citations

Symmetry recognition applied to two-dimensional NMR data

1987 • 42 citations

Multivariate data analysis for pattern recognition in two-dimensional NMR

1988 • 38 citations

PARIS, a program for automatic recognition and integration of 2D NMR signals

1989 • 31 citations

PROSPECT, a program for automated interpretation of 2D NMR spectra of proteins

1990 • 31 citations

Automated elucidation of J connectivities in 1H NMR spectra

1989 • 26 citations

Toward automatic assignment of protein 1H NMR spectra

1989 • 25 citations

Pepto: An expert system for automatic peak assignment of two‐dimensional nuclear magnetic resonance spectra of proteins

1990 • 23 citations

High-level expression of recombinant human FK-binding protein from a fusion precursor

1992 • 22 citations

High-level synthesis of the 12-kDa human FK506-binding protein in Escherichia coli using translational coupling

1993 • 17 citations