Human beta-hexosaminidase alpha chain: coding sequence and homology with the beta chain.

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

1977 • 12,015 citations

Rapid and Sensitive Protein Similarity Searches

1985 • 4,091 citations

The Metabolic Basis of Inherited Disease.

1983 • 2,885 citations

Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.

1983 • 2,456 citations

Patterns of Amino Acids near Signal‐Sequence Cleavage Sites

1983 • 2,290 citations

Lysosomes in biology and pathology

1977 • 2,068 citations

Rapid similarity searches of nucleic acid and protein data banks.

1983 • 1,577 citations

A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides

1983 • 1,143 citations

Plasmid screening at high colony density

1980 • 1,100 citations

A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.

1983 • 933 citations

A reliable method for the recovery of DNA fragments from agarose and acrylamide gels

1981 • 916 citations

Biosynthesis of lysosomal enzymes in fibroblasts. Synthesis as precursors of higher molecular weight.

1980 • 809 citations

Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.

1980 • 570 citations

THE PATHWAY OF EUKARYOTIC mRNA FORMATION

1983 • 429 citations

Recognition and receptor-mediated uptake of a lysosomal enzyme, α-l-iduronidase, by cultured human fibroblasts

1977 • 354 citations

Homology of amino acid sequences of rat liver cathepsins B and H with that of papain.

1983 • 231 citations

Evidence for two different active sites on human beta-hexosaminidase A. Interaction of GM2 activator protein with beta-hexosaminidase A.

1985 • 223 citations

Mapping the Human Genome, Cloned Genes, DNA Polymorphisms, and Inherited Disease

1982 • 197 citations

Rapid purification of plasmid DNA by a single centrifugation in a two-step cesium chloride-ethidium bromide gradient

1983 • 161 citations

Association of alpha- and beta-subunits during the biosynthesis of beta-hexosaminidase in cultured human fibroblasts.

1984 • 159 citations

Lysosomal enzyme phosphorylation. Recognition of a protein-dependent determinant allows specific phosphorylation of oligosaccharides present on lysosomal enzymes.

1984 • 150 citations

Interleukin 2 (IL2) is assigned to human chromosome 4

1984 • 143 citations

Isolation of a cDNA clone for human antithrombin III.

1983 • 134 citations

Assignment of the β-glucuronidase structural gene to the pter→q22 region of chromosome 7 in man

1978 • 117 citations

Isolation of cDNA clones coding for the beta subunit of human beta-hexosaminidase.

1985 • 116 citations

Synthesis of beta-hexosaminidase in cell-free translation and in intact fibroblasts: an insoluble precursor alpha chain in a rare form of Tay-Sachs disease.

1982 • 113 citations

Tay-Sachs' and Sandhoff's diseases: the assignment of genes for hexosaminidase A and B to individual human chromosomes.

1975 • 108 citations

Human β-D- N -Acetylhexosaminidases A and B: Expression and Linkage Relationships in Somatic Cell Hybrids

1974 • 100 citations

Isolation of human fibroblast catalase cDNA clones. Sequence of clones derived from spliced and unspliced mRNA.

1984 • 90 citations

UDP-N-acetylglucosamine:lysosomal enzyme precursor N-acetylglucosamine-1-phosphotransferase. Partial purification and characterization of the rat liver Golgi enzyme.

1982 • 85 citations

Molecular cloning of a cDNA for human alpha-L-fucosidase.

1985 • 78 citations

Identification of cDNA clones encoding a precursor of rat liver cathepsin B.

1985 • 73 citations

cDNA clone for the alpha-chain of human beta-hexosaminidase: deficiency of alpha-chain mRNA in Ashkenazi Tay-Sachs fibroblasts.

1984 • 64 citations

The Biochemical Basis of Gangliosidoses

1984 • 59 citations

Heterogeneity in type I Gaucher disease demonstrated by restriction mapping of the gene.

1985 • 46 citations

Amino acid sequence of porcine spleen cathepsin D light chain.

1983 • 29 citations

Isolation and expression in Escherichia coli of a cDNA clone encoding human β-glucuronidase

1985 • 26 citations