Nucleotide sequence of the gene for aqualysin I (a thermophilic alkaline serine protease) of Thermus aquaticus YT‐1 and characteristics of the deduced primary structure of the enzyme

A procedure for the isolation of deoxyribonucleic acid from micro-organisms

1961 • 11,554 citations

Studies on transformation of Escherichia coli with plasmids

1983 • 11,179 citations

The Preparation and Enzymatic Hydrolysis of Reduced and S-Carboxymethylated Proteins

1963 • 2,600 citations

Dideoxy sequencing method using denatured plasmid templates

1986 • 1,537 citations

Plasmid screening at high colony density

1980 • 1,100 citations

Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP

1986 • 717 citations

Thermus aquaticus gen. n. and sp. n., a Nonsporulating Extreme Thermophile

1969 • 675 citations

Gene structure and extracellular secretion of Neisseria gonorrhoeae IgA protease

1987 • 634 citations

High guanine plus cytosine content in the third letter of codons of an extreme thermophile. DNA sequence of the isopropylmalate dehydrogenase of Thermus thermophilus.

1984 • 207 citations

A method for the determination of amino acid sequence in peptides.

1949 • 196 citations

Subtilisin Carlsberg

1968 • 194 citations

On the Hydrophobic Nature of Signal Sequences

1981 • 193 citations

Ciliated epithelium in a cyst of the lower limb

1969 • 174 citations

X-ray crystallographic study of the binding of peptide chloromethyl ketone inhibitors to subtilisin BPN

1972 • 168 citations

Specific excretion of Serratia marcescens protease through the outer membrane of Escherichia coli

1986 • 159 citations

16 Subtilisins: Primary Structure, Chemical and Physical Properties

1971 • 155 citations

Subtilisin BPN'

1967 • 124 citations

Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin‐type proteinases

1985 • 114 citations

Amino acid sequence of proteinase K from the mold Tritirachium album Limber

1986 • 114 citations

Purification and characterization of aqualysin I (a thermophilic alkaline serine protease) produced by Thermus aquaticus YT‐1

1988 • 110 citations

Nucleotide sequence of the malate dehydrogenase gene of Thermus flavus and its mutation directing an increase in enzyme activity.

1986 • 100 citations

Purification and some properties of an extracellular protease (caldolysin) from an extreme thermophile

1982 • 90 citations

Three-dimensional structure of fungal proteinase K reveals similarity to bacterial subtilisin.

1984 • 85 citations

Nucleotide sequence and characteristics of the gene for <scp>l</scp>‐lactate dehydrogenase of Thermus caldophilus GK24 and the deduced amino‐acid sequence of the enzyme

1986 • 58 citations

Subtilisin Amylosacchariticus

1972 • 55 citations

Determination of the Complete Amino-Acid Sequence of Subtilisin DY and its Comparison with the Primary Structures of the Subtilisins BPN’, Carlsberg and Amylosacchariticus

1985 • 37 citations

Production of thermophilic extracellular proteases (aqualysins I and II) by Thermus aquaticus YT-1, an extreme thermophile.

1983 • 37 citations

A novel method for selective isolation of C‐terminal peptides from tryptic digests of proteins by immobilized anhydrotrypsin: Application to structural analyses of the tail sheath and tube proteins from bacteriophage T4

1986 • 27 citations

Electron Microscopic Study on the Structures Formed by Mixtures Containing Synthetic Glyceroglycolipids1

1983 • 24 citations

15 Subtilisin: X-Ray Structure

1971 • 23 citations