Construction of a Yeast Strain Deleted for theTRP1 Promoter and Coding Region that Enhances the Efficiency of the Polymerase Chain Reaction-Disruption Method

New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiae

1994 • 2,554 citations

Elevated recombination rates in transcriptionally active DNA

1989 • 1,666 citations

A simple and efficient method for direct gene deletion inSaccharomyces cerevisiae

1993 • 1,256 citations

A family of low and high copy replicative, integrative and single‐stranded S. cerevisiae/E. coli shuttle vectors

1991 • 533 citations

Naturally occurring poly(dA-dT) sequences are upstream promoter elements for constitutive transcription in yeast.

1985 • 526 citations

Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene

1980 • 511 citations

Functional Requirement of a Site-Specific Ribose Methylation in Ribosomal RNA

1993 • 129 citations

Functional analysis reports. Precise gene disruption in Saccharomyces cerevisiae by double fusion polymerase chain reaction

1995 • 126 citations

A method for performing precise alterations in the yeast genome using a recyclable selectable marker

1995 • 98 citations

Expression inSaccharomyces cerevisiaeof human interferon-alpha directed by theTRP1 5′region

1983 • 54 citations