Isolation and characterization of a novel yeast gene, ATH1, that is required for vacuolar acid trehalase activity

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

Experiments in molecular genetics

1972 • 28,260 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

1979 • 22,482 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

A comprehensive set of sequence analysis programs for the VAX

1984 • 14,824 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

A new method for predicting signal sequence cleavage sites

1986 • 4,629 citations

The scanning model for translation: an update.

1989 • 3,525 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

Analysis of gene control signals by DNA fusion and cloning in Escherichia coli

1980 • 2,499 citations

Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase

1982 • 1,539 citations

Methods in yeast genetics, laboratory manual

1987 • 733 citations

Stationary phase in the yeast Saccharomyces cerevisiae

1993 • 594 citations

Trehalose in yeast, stress protectant rather than reserve carbohydrate

1990 • 537 citations

A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts

1946 • 530 citations

The Metabolism of α,α-Trehalose

1974 • 526 citations

Regulation of trehalose mobilization in fungi

1984 • 480 citations

Aminopeptidase I of Saccharomyces cerevisiae is localized to the vacuole independent of the secretory pathway.

1992 • 371 citations

One-Step Gene Disruption in Yeast

1989 • 310 citations

The amino terminus of the yeast F1-ATPase beta-subunit precursor functions as a mitochondrial import signal.

1986 • 258 citations

Yeast promoters: Positive and negative elements

1984 • 227 citations

Gene dosage-dependent secretion of yeast vacuolar carboxypeptidase Y.

1986 • 217 citations

Vacuoles: The sole compartments of digestive enzymes in yeast (Saccharomyces cerevisiae)?

1979 • 202 citations

Inactivation of YME1, a member of the ftsH-SEC18-PAS1-CDC48 family of putative ATPase-encoding genes, causes increased escape of DNA from mitochondria in Saccharomyces cerevisiae.

1993 • 199 citations

Differential importance of trehalose in stress resistance in fermenting and nonfermenting Saccharomyces cerevisiae cells

1995 • 188 citations

7 General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae

1992 • 167 citations

Trehalose inhibits ethanol effects on intact yeast cells and liposomes

1994 • 159 citations

Cloning of two related genes encoding the 56-kDa and 123-kDa subunits of trehalose synthase from the yeast Saccharomyces cerevisiae

1993 • 156 citations

Overproduction-induced mislocalization of a yeast vacuolar protein allows isolation of its structural gene.

1986 • 151 citations

Molecular analysis of the neutral trehalase gene from Saccharomyces cerevisiae.

1993 • 144 citations

Characterization of two trehalases in baker's yeast

1984 • 139 citations

Trehalose levels and survival ratio of freeze-tolerant versus freeze-sensitive yeasts

1990 • 130 citations

Purification and Characterization of Neutral Trehalase from the Yeast ABYS1 Mutant

1989 • 103 citations

[16] Gene overexpression in studies of Saccharomyces cerevisiae

1991 • 102 citations

Purification and characterization of acid trehalase from the yeast suc2 mutant.

1988 • 95 citations

Analysis and DNA sequence of the osmoregulatedtreA gene encoding the periplasmic trehalase ofEscherichia coli K12

1989 • 81 citations

Metabolic regulation of the trehalose content of vegetative yeast

1991 • 81 citations

Trehalase from male accessory gland of an insect, Tenebrio molitor. cDNA sequencing and developmental profile of the gene expression

1992 • 73 citations

Rabbit small intestinal trehalase. Purification, cDNA cloning, expression, and verification of glycosylphosphatidylinositol anchoring.

1990 • 70 citations

Identification and characterization of a novel yeast gene: the YGP1 gene product is a highly glycosylated secreted protein that is synthesized in response to nutrient limitation.

1994 • 70 citations

Selection of Yeasts for Breadmaking by the Frozen-Dough Method

1986 • 67 citations

Effect of Growth Conditions and Trehalose Content on Cryotolerance of Bakers' Yeast in Frozen Doughs

1989 • 67 citations

Is thermotolerance of yeast dependent on trehalose accumulation?

1994 • 60 citations

Purification, cDNA cloning and Northern blot analysis of trehalase of pupal midgut of the silkworm, Bombyx mori

1993 • 51 citations

The need for consistent nomenclature and assessment of growth phases in diauxic cultures of Saccharomyces cerevisiae

1993 • 47 citations

Differential changes in the activity of cytosolic and vacuolar trehalases along the growth cycle of Saccharomyces cerevisiae

1994 • 43 citations

Heat‐shock response in a yeast tps1 mutant deficient in trehalose synthesis

1994 • 29 citations

Vacuolar (lysosomal) trehalase ofSaccharomyces cerevisiae

1987 • 23 citations

Transport of yeast vacuolar trehalase to the vacuole

1988 • 16 citations