Improving structural integrity of cryosections for immunogold labeling

Chapter 4 Preparation of Isolated Rat Liver Cells

1976 • 5,335 citations

A new method of preparing gold probes for multiple-labeling cytochemistry.

1985 • 1,417 citations

A TECHNIQUE FOR ULTRACRYOTOMY OF CELL SUSPENSIONS AND TISSUES

1973 • 1,057 citations

Ultrastructural localization of intracellular antigens by the use of protein A-gold complex.

1978 • 992 citations

Immuno-localization of the insulin regulatable glucose transporter in brown adipose tissue of the rat.

1991 • 874 citations

INTRACELLULAR TRANSPORT OF SECRETORY PROTEINS IN THE PANCREATIC EXOCRINE CELL

1967 • 841 citations

Fine Structure Immunocytochemistry

1993 • 707 citations

Segregation of MHC class II molecules from MHC class I molecules in the Golgi complex for transport to lysosomal compartments

1991 • 663 citations

Application of cryoultramicrotomy to immunocytochemistry

1986 • 516 citations

On the preparation of cryosections for immunocytochemistry

1984 • 468 citations

The biosynthetic pathway of MHC class II but not class I molecules intersects the endocytic route

1990 • 445 citations

Movement of internalized ligand–receptor complexes along a continuous endosomal reticulum

1990 • 428 citations

Use of poly(vinylpyrrolidone) and poly(vinyl alcohol) for cryoultramicrotomy

1989 • 403 citations

A study of positive staining of ultrathin frozen sections

1978 • 329 citations

High pressure freezing comes of age.

1989 • 324 citations

Improved procedures for immunoferritin labeling of ultrathin frozen sections.

1976 • 283 citations

Liposome-encapsulated antigens are processed in lysosomes, recycled, and presented to T cells

1991 • 250 citations

Electron microscopy of frozen-hydrated bacteria

1983 • 244 citations

FROZEN THIN SECTIONS OF FRESH TISSUE FOR ELECTRON MICROSCOPY, WITH A DESCRIPTION OF PANCREAS AND LIVER

1971 • 217 citations

An acidic sequence within the cytoplasmic domain of furin functions as a determinant of trans-Golgi network localization and internalization from the cell surface.

1995 • 215 citations

Mammalian Sec23p homologue is restricted to the endoplasmic reticulum transitional cytoplasm.

1991 • 196 citations

Intracellular localization of the copper-zinc and manganese superoxide dismutases in rat liver parenchymal cells.

1986 • 195 citations

Beta-COP localizes mainly to the cis-Golgi side in exocrine pancreas.

1993 • 190 citations

Synthesis and Assembly of Lung Surfactant

1991 • 141 citations

Subcellular localization of Forssman glycolipid in epithelial MDCK cells by immuno-electronmicroscopy after freeze-substitution.

1991 • 134 citations

Ultrastructural and Immunocytochemical Characterization of Autophagic Vacuoles in Isolated Hepatocytes: Effects of Vinblastine and Asparagine on Vacuole Distributions

1995 • 124 citations

Surfactant protein A is localized at the corners of the pulmonary tubular myelin lattice.

1991 • 103 citations

ULTRATHIN FROZEN SECTIONS

1967 • 102 citations

Immunocytochemical localization of amylase and chymotrypsinogen in the exocrine pancreatic cell with special attention to the Golgi complex.

1979 • 95 citations

Bacterial mesosomes: Method dependent artifacts

1981 • 93 citations

Immunoferritin Localization of Intracellular Antigens: The Use of Ultracryotomy to Obtain Ultrathin Sections Suitable for Direct Immunoferritin Staining

1973 • 89 citations

Immunoelectron microscopic exploration of the Golgi complex.

1983 • 79 citations

Distribution of CuZn superoxide dismutase in rat liver

1993 • 76 citations

The biogenesis of the MHC class II compartment in human I-cell disease B lymphoblasts.

1996 • 72 citations

Function of coated membranes and multivesicular bodies during membrane regulation in stimulated exocrine pancreas cells

1974 • 71 citations

The fixative action of uranyl acetate in electron microscopy

1968 • 69 citations

Usefulness of the immunogold technique in quantitation of a soluble protein in ultra-thin sections.

1987 • 65 citations

Uranyl salts as fixatives for electron microscopy

1971 • 65 citations

A three-dimensional reconstruction study of the rough ER-Golgi interface in serial thin sections of the pancreatic acinar cell of the rat

1994 • 65 citations

Disproportional immunostaining patterns of two secretory proteins in guinea pig and rat exocrine pancreatic cells. An immunoferritin and fluorescence study.

1980 • 57 citations

The differential degradation of two cytosolic proteins as a tool to monitor autophagy in hepatocytes by immunocytochemistry.

1993 • 53 citations

Immunohistochemistry

2002 • 52 citations

Rapid-freezing cytochemistry: preservation of tubular lysosomes and enzyme activity.

1991 • 40 citations

Intramembrane particles and filipin labelling on the membranes of autophagic vacuoles and lysosomes in mouse liver

1989 • 35 citations

Cryosubstitution dehydration of aldehyde-fixed tissue: a favorable approach to quantitative immunocytochemistry.

1994 • 33 citations

Fixation for Fine Structure Preservation and Immunocytochemistry

1993 • 21 citations

Albumin localization in rat liver parenchymal cells.

1983 • 19 citations

Preservation and immunogold localization of lipids by freeze-substitution and low temperature embedding.

1991 • 18 citations

Composition of the transfer medium is crucial for high-resolution immunocytochemistry of cryosectioned human neutrophils.

1994 • 16 citations

Use of electrical methods in the study of hepatocytic autophagy.

1986 • 13 citations

Ultracryotomy of nerve-electroplaque synapses for immunocytochemistry

1978 • 10 citations

Ultrathin cryosections in the plane of cell monolayers: Evaluation of their potential for antibody localization studies of the cytoskeleton

1988 • 9 citations

[ULTRAMICROTOMY AT LOW TEMPERATURE].

1965 • 5 citations

Cryoultramicrotomy: Electrostatic Transfer of Dry Ultrathin Frozen Sections on Grids Applied to the Central Nervous System.

1992 • 5 citations

Expression of Functional Growth Hormone Receptor in a Mouse L Cell Line Infected with Recombinant Vaccinia Virus

1994 • 4 citations

Attempted direct visualization of negatively stained amplified immune complex of synaptic acetylcholinesterase using cryoultramicrotomy sections

1981 • 3 citations