Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis: a blind comparison study among seven laboratories

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Rapid and simple method for purification of nucleic acids

1990 • 5,273 citations

Avoiding false positives with PCR

1989 • 3,564 citations

Tuberculosis: Commentary on a Reemergent Killer

1992 • 1,400 citations

Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis

1991 • 996 citations

Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions

1990 • 953 citations

Polymerase Chain Reaction Amplification of a Repetitive DNA Sequence Specific for Mycobacterium tuberculosis

1990 • 763 citations

Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications

1992 • 756 citations

Detection and identification of mycobacteria by amplification of rRNA

1990 • 523 citations

Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis

1990 • 483 citations

IS6110, an IS-like element ofMycobacterium tuberculosiscomplex

1990 • 441 citations

Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis

1990 • 419 citations

RAPID DIAGNOSIS OF TUBERCULOSIS BY AMPLIFICATION OF MYCOBACTERIAL DNA IN CLINICAL SAMPLES

1989 • 406 citations

Insertion element IS987 from Mycobacterium bovis BCG is located in a hot-spot integration region for insertion elements in Mycobacterium tuberculosis complex strains

1991 • 399 citations

Detection ofMycobacterium tuberculosisin Sputum Samples Using a Polymerase Chain Reaction

1991 • 364 citations

Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine mycobacteriology laboratory

1993 • 346 citations

Detection and identification of mycobacteria by amplification of mycobacterial DNA

1989 • 313 citations

Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation

1991 • 309 citations

Rapid diagnosis of tuberculous meningitis by polymerase chain reaction

1991 • 253 citations

Specific detection of Mycobacterium tuberculosis complex strains by polymerase chain reaction

1990 • 233 citations

Direct detection of Mycobacterium tuberculosis in clinical specimens by DNA amplification

1990 • 222 citations

Detection of Mycobacterium tuberculosis in clinical samples by using polymerase chain reaction and a nonradioactive detection system

1992 • 222 citations

Detection and identification of Mycobacterium tuberculosis by DNA amplification

1990 • 216 citations

Amplification of a species-specific DNA fragment of Mycobacterium tuberculosis and its possible use in diagnosis

1991 • 203 citations

Polymerase chain reaction for detection of Mycobacterium tuberculosis

1990 • 198 citations

Use of polymerase chain reaction for rapid diagnosis of tuberculosis

1992 • 189 citations

Characterization of a Mycobacterium tuberculosis insertion sequence belonging to the IS3 family

1990 • 188 citations

Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction and DNA hybridization

1993 • 183 citations

Progress toward a simplified polymerase chain reaction and its application to diagnosis of tuberculosis

1993 • 157 citations

Direct detection of Mycobacterium tuberculosis in respiratory specimens in a clinical laboratory by polymerase chain reaction

1993 • 157 citations

Use of a reamplification protocol improves sensitivity of detection of Mycobacterium tuberculosis in clinical samples by amplification of DNA

1991 • 151 citations

Detection by polymerase chain reaction of Treponema pallidum DNA in cerebrospinal fluid from neurosyphilis patients before and after antibiotic treatment

1991 • 135 citations

Evaluation of a polymerase chain reaction for the diagnosis of tuberculosis

1991 • 130 citations

A simple method for diagnosing M. tuberculosis infection in clinical samples using PCR

1991 • 128 citations

Detection of Mycobacterium tuberculosis in clinical samples by two-step polymerase chain reaction and nonisotopic hybridization methods

1993 • 121 citations

Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples

1993 • 113 citations

Rapid, simple method for treating clinical specimens containing Mycobacterium tuberculosis to remove DNA for polymerase chain reaction

1992 • 103 citations

Rapid diagnosis Of tuberculous meningitis by polymerase chain reaction (PCR)

1990 • 96 citations

Use of gene probes based on the insertion sequence IS986 to differentiate between BCG vaccine strains

1992 • 81 citations

Detection of Mycobacterium tuberculosis DNA in clinical samples by using a simple lysis method and polymerase chain reaction

1993 • 75 citations

Genus- and species-specific DNA probes to identify mycobacteria using the polymerase chain reaction

1990 • 58 citations

Simplified colorimetric analysis of polymerase chain reactions: detection of HIV sequences in AIDS patients

1990 • 40 citations

Differentiation of Mycobacterium tuberculosis and Mycobacterium bovis BCG by a polymerase chain reaction assay

1991 • 35 citations

Enzyme immunoassay for identification of heat-killed mycobacteria belonging to the Mycobacterium tuberculosis and Mycobacterium avium complexes and derived from early cultures

1990 • 27 citations

Simple method for production of internal control DNA for Mycobacterium tuberculosis polymerase chain reaction assays

1993 • 22 citations

The detection of Mycobacterium tuberculosis in uncultured clinical specimens using the polymerase chain reaction and a non-radioactive DNA probe

1992 • 20 citations