Molecular cloning of an endoglucanase gene from an alkalophilic Bacillus sp. and its expression in Escherichia coli

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

1992 • 4,683 citations

Preparation of transforming deoxyribonucleic acid by phenol treatment

1963 • 1,813 citations

High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

1979 • 1,504 citations

The Release of Enzymes from Escherichia coli by Osmotic Shock and during the Formation of Spheroplasts

1965 • 1,490 citations

Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.

1983 • 1,075 citations

Measurement of carboxymethylcellulase activity

1960 • 820 citations

Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid

1975 • 449 citations

Factors affecting the transformation of Escherichia coli strain χ1776 by pBR322 plasmid DNA

1978 • 171 citations

Characterization of Two Cel (Cellulose Degradation) Genes of Clostridium Thermocellum Coding for Endoglucanases

1983 • 135 citations

Molecular cloning of a Cellulomonas fimi cellulase gene in Escherichia coli

1982 • 116 citations

Molecular cloning and expression of a Bacillus subtilis β-glucanase gene in Escherichia coli

1983 • 93 citations

Cloning and expression in Escherichia coli of Clostridium thermocellum genes coding for amino acid synthesis and cellulose hydrolysis

1983 • 92 citations

Molecular cloning of a Bacillus subtilis xylanase gene in Escherichia coli

1983 • 84 citations

Molecular cloning and expression of cellulase genes of alkalophilic Bacillus sp. strain N-4 in Escherichia coli

1984 • 76 citations

Cloning and Expression of a Thermomonospora YX Endocellulase Gene in E. coli

1983 • 69 citations

Biological expression of an Escherichia coli consensus sequence promoter and some mutant derivatives.

1983 • 46 citations

Properties of Bacillus cereus spore coat mutants

1975 • 43 citations

Molecular cloning of a cellulase gene from Bacillus subtilis and its expression in Escherichia coli.

1986 • 27 citations

Isolation of Endoglucanase Genes from Pseudomonas fluorescens subsp. cellulosa and a Pseudomonas sp

1986 • 20 citations