Development of an aqueous-space mixing assay for fusion of granules and plasma membranes from human neutrophils

Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

1968 • 5,084 citations

Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate

1983 • 2,596 citations

Use of resonance energy transfer to monitor membrane fusion

1981 • 1,274 citations

Subcellular localization of the b-cytochrome component of the human neutrophil microbicidal oxidase: translocation during activation.

1983 • 938 citations

Fluorescence method for measuring the kinetics of fusion between biological membranes

1984 • 670 citations

Studies on the mechanism of membrane fusion: kinetics of calcium ion induced fusion of phosphatidylserine vesicles followed by a new assay for mixing of aqueous vesicle contents

1980 • 488 citations

Proton- and calcium-induced fusion and destabilization of liposomes

1985 • 471 citations

Laboratory Manual of Neutrophil Function

1986 • 443 citations

Aggregation of chromaffin granules by calpactin at micromolar levels of calcium

1988 • 443 citations

Five distinct calcium and phospholipid binding proteins share homology with lipocortin I.

1988 • 364 citations

A simple method for the accurate determination of free [Ca] in Ca-EGTA solutions

1982 • 331 citations

Characterization of Ca2+-dependent phospholipid binding, vesicle aggregation and membrane fusion by annexins

1990 • 293 citations

cis-Unsaturated fatty acids induce the fusion of chromaffin granules aggregated by synexin.

1981 • 272 citations

Ca2+ homeostasis in permeabilized human neutrophils. Characterization of Ca2+-sequestering pools and the action of inositol 1,4,5-triphosphate.

1984 • 266 citations

Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport

1989 • 250 citations

Ca2+-induced fusion of phospholipid vesicles monitored by mixing of aqueous contents

1979 • 239 citations

Intracellular Free Calcium Localization in Neutrophils During Phagocytosis

1985 • 217 citations

Proton-induced fusion of oleic acid-phosphatidylethanolamine liposomes

1985 • 204 citations

Chemotactic factor causes rapid decreases in phosphatidylinositol,4,5-bisphosphate and phosphatidylinositol 4-monophosphate in rabbit neutrophils

1983 • 188 citations

Possible Synthesis of Polyribonucleotides of Known Base-triplet Sequences

1965 • 180 citations

Release of Ca2+ from a non-mitochondrial store site in peritoneal macrophages treated with saponin by inositol 1,4,5-trisphosphate

1984 • 171 citations

Separation and characterization of human neutrophil granules.

1974 • 163 citations

Calcium-induced lysozyme secretion from human polymorphonuclear leukocytes

1974 • 148 citations

An enzyme-linked immunoassay (ELISA) for measurement of lactoferrin

1983 • 113 citations

Synexin facilitates fusion of specific phospholipid membranes at divalent cation concentrations found intracellularly.

1982 • 69 citations

Fusogenic capacities of divalent cations and effect of liposome size

1985 • 63 citations

Coupling of polyphosphoinositide breakdown with calcium efflux in formyl‐methionyl‐leucyl‐phenylalanine‐stimulated rabbit neutrophils

1983 • 60 citations

Synexin-like proteins from human polymorphonuclear leukocytes. Identification and characterization of granule-aggregating and membrane-fusing activities.

1987 • 57 citations

Annexin I interactions with human neutrophil specific granules: fusogenicity and coaggregation with plasma membrane vesicles

1993 • 48 citations

Membrane fusion without cytoplasmic fusion (hemi-fusion) in erythrocytes that are subjected to electrical breakdown

1991 • 46 citations

Relationship between arachidonic acid release and Ca2+-dependent exocytosis in digitonin-permeabilized bovine adrenal chromaffin cells

1990 • 42 citations

Plasma membrane-derived vesicles containing receptor-ligand complexes are fusogenic with early endosomes in a cell-free system.

1988 • 41 citations

Modeling degranulation with liposomes: Effect of lipid composition on membrane fusion

1994 • 19 citations

Calcium-dependent fusion of the plasma membrane fraction from human neutrophils with liposomes

1990 • 17 citations

Isolation of Mononuclear Leukocytes in a Plastic Bag System Using Ficoll-Hypaque

1985 • 11 citations