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Heating RNA before cell-free translation is essential for the efficient and reproducible synthesis of several peroxisomal proteins

Data up to Jan 2025

Published1984
Citations11
References15

Total Citations Per Year

Abstract

References (15)

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

1979 • 22,482 citations

Quantitative Film Detection of 3H and 14C in Polyacrylamide Gels by Fluorography

1975 • 4,693 citations

An Efficient mRNA‐Dependent Translation System from Reticulocyte Lysates

1976 • 3,994 citations

Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.

1982 • 1,765 citations

Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences

1977 • 1,252 citations

Rat liver peroxisomes catalyze the beta oxidation of fatty acids.

1978 • 608 citations

NATURE OF THE HEPATOMEGALIC EFFECT PRODUCED BY ETHYL-CHLOROPHENOXY-ISOBUTYRATE IN THE RAT

1965 • 599 citations

Methylmercury hydroxide enhancement of translation and transcription of ovalbumin and conalbumin mRNA's.

1979 • 175 citations

Rat liver pre-proalbumin: complete amino acid sequence of the pre-piece. Analysis of the direct translation product of albumin messenger RNA.

1977 • 135 citations

Synthesis of catalase in two cell-free protein-synthesizing systems and in rat liver

1978 • 121 citations

Two fractions of rough endoplasmic reticulum from rat liver. II. Cytoplasmic messenger RNA's which code for albumin and mitochondrial proteins are distributed differently between the two fractions.

1977 • 104 citations

Mechanism of compartmentation of secretory proteins: transport of exocrine pancreatic proteins across the microsomal membrane

1980 • 99 citations

Rat liver preproalbumin: in vitro synthesis and partial amino acid sequence.

1977 • 84 citations

Stimulation of in Vitro Translation of Messenger RNA by Actinomycin D and Cordycepin

1977 • 41 citations

Indentification of β‐oxidation enzymes among peroxisomal polypeptides

1982 • 22 citations

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Heating RNA before cell-free translation is essential for the efficient and reproducible… (1984) – Biochemical Journal | Metascience Observatory Explorer