The preparation and screening of a cDNA clone bank

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Experiments in molecular genetics

1972 • 28,260 citations

Continuous cultures of fused cells secreting antibody of predefined specificity

1975 • 17,037 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

A new method for sequencing DNA.

1977 • 7,941 citations

Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose

1972 • 6,922 citations

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

1992 • 4,683 citations

A membrane-filter technique for the detection of complementary DNA

1966 • 3,996 citations

A complementation analysis of the restriction and modification of DNA in Escherichia coli

1969 • 3,866 citations

Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

1977 • 3,829 citations

Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

1975 • 3,483 citations

Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids

1977 • 3,019 citations

Calcium-dependent bacteriophage DNA infection

1970 • 2,743 citations

Ribonucleic acid isolated by cesium chloride centrifugation

1974 • 2,263 citations

Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

1977 • 2,233 citations

Viral RNA-dependent DNA Polymerase: RNA-dependent DNA Polymerase in Virions of Rous Sarcoma Virus

1970 • 2,061 citations

Viral RNA-dependent DNA Polymerase: RNA-dependent DNA Polymerase in Virions of RNA Tumour Viruses

1970 • 1,992 citations

A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane

1965 • 1,981 citations

Number and evolutionary conservation of α- and β-tubulin and cytoplasmic β- and γ-actin genes using specific cloned cDNA probes

1980 • 1,884 citations

The isolation of structural genes from libraries of eucaryotic DNA

1978 • 1,877 citations

Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells

1979 • 1,454 citations

Determinant of cistron specificity in bacterial ribosomes

1975 • 1,363 citations

Methylmercury as a reversible denaturing agent for agarose gel electrophoresis

1976 • 1,323 citations

A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome

1976 • 1,313 citations

Mapping of RNA by a modification of the Berk-Sharp procedure: the 5′ termini of 15 S β-globin mRNA precursor and mature 10 S β-globin mRNA have identical map coordinates

1979 • 1,286 citations

Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences

1977 • 1,252 citations

Plasmid screening at high colony density

1980 • 1,100 citations

Trans-complementable copy-number mutants of plasmid ColE1

1980 • 975 citations

Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.

1978 • 972 citations

Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin

1977 • 829 citations

Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.

1978 • 791 citations

Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing Lambda Phage Genes and the Galactose Operon of Escherichia coli

1972 • 723 citations

Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide

1977 • 713 citations

Three abundance classes in HeLa cell messenger RNA

1974 • 563 citations

Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone

1977 • 532 citations

Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone

1979 • 517 citations

A bacterial clone synthesizing proinsulin.

1978 • 515 citations

Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.

1979 • 501 citations

Amplification and characterization of a β-globin gene synthesized in vitro

1976 • 495 citations

Synthesis in E. coli of a polypeptide with human leukocyte interferon activity

1980 • 484 citations

A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster

1974 • 481 citations

Inhibition of intractable, nucleases with ribonucleoside-vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytes

1979 • 461 citations

The primary structure of rabbit β-globin mRNA as determined from cloned DNA

1977 • 428 citations

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

1978 • 426 citations

Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynudeotidyl transferase

1976 • 416 citations

Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.

1978 • 395 citations

Adenine-rich Polymer associated with Rabbit Reticulocyte Messenger RNA

1970 • 355 citations

Enzymatic in vitro synthesis of globin genes

1976 • 343 citations

Enzymatic end-to-end joining of DNA molecules

1973 • 311 citations

Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit

1976 • 307 citations

Structural gene sets active in embryos and adult tissues of the sea urchin

1976 • 298 citations

Synthesis of full length cDNAs from four partially purified oviduct mRNAs.

1978 • 279 citations

Splicing and the formation of stable RNA

1979 • 274 citations

Biochemical method for mapping mutational alterations in DNA with S1 nuclease: the location of deletions and temperature-sensitive mutations in simian virus 40.

1975 • 264 citations

Regulation of structural gene expression in tobacco

1980 • 262 citations

Expression in Escherichia coli of hepatitis B virus DNA sequences cloned in plasmid pBR322

1979 • 239 citations

In vitro Synthesis of DNA Complementary to Rabbit Reticulocyte 10S RNA

1972 • 234 citations

Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit β-globin

1980 • 227 citations

Rapid Purification of Plasmid DNAs by Hydroxyapatite Chromatography

1978 • 225 citations

Full length and discrete partial reverse transcripts of globin and chorion mRNAs

1975 • 223 citations

Synthetic lacoperator DNA is functional in vivo

1976 • 196 citations

In Vitro Synthesis of DNA Complementary to Purified Rabbit Globin mRNA

1972 • 192 citations

Transcription of the Vaccinia Virus Genome and the Occurrence of Polyriboadenylic Acid Sequences in Messenger RNA

1970 • 187 citations

Splicing as a requirement for biogenesis of functional 16S mRNA of simian virus 40.

1979 • 185 citations

Nucleotide sequence analysis of the leader segments in a cloned copy of adenovirus 2 fiber mRNA

1979 • 184 citations

Nucleic acid hybridization using DNA covalently coupled to cellulose

1975 • 182 citations

In vitro Synthesis of DNA Components of Human Genes for Globins

1972 • 173 citations

Isolation of Hen Oviduct Ovalbumin and Rat Liver Albumin Polysomes by Indirect Immunoprecipitation

1974 • 172 citations

Factors affecting the transformation of Escherichia coli strain χ1776 by pBR322 plasmid DNA

1978 • 171 citations

Insertion of a rabbit β-globin gene sequence into an E.coli plasmid

1975 • 164 citations

Immunological screening method to detect specific translation products.

1978 • 160 citations

Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.

1976 • 137 citations

Secondary structure of mouse and rabbit α- and β-globin mRNAs: Differential accessibility of α and β initiator AUG codons towards nucleases

1980 • 133 citations

Construction and selection of recombinant plasmids containing full-length complementary DNAs corresponding to rat insulins I and II.

1979 • 110 citations

Changes in the abundance of polyadenylated RNA during slime mould development measured using cloned molecular hybridization probes

1979 • 105 citations

A general method for cloning eukaryotic structural gene sequences.

1976 • 100 citations

Chicken ovalbumin is synthesized and secreted by Escherichia coli.

1978 • 100 citations

Molecular cloning and sequence analysis of adult chicken β globin cDNA

1979 • 95 citations

Fractionation and functional analysis of newly synthesized and decaying messenger RNAs from vegetative cells of Dictyostelium discoideum

1979 • 94 citations

Characterization and transcription analysis of a cloned sequence derived from a major developmentally regulated mRNA of D. discoideum

1979 • 94 citations

The amino-terminal sequence of human fibroblast interferon as deduced from reverse transcripts obtained using synthetic oligonucleotide primers

1980 • 93 citations

Quantitative analysis of specific labelled RNA's using DNA covalently linked to diazobenzyloxy-methyl-paper

1979 • 87 citations

A sensitive radioimmunoassay for detecting products translated from cloned DNA fragments

1978 • 81 citations

Characterisation of bacterial clones containing DNA sequences derived from Xenopus laevis vitellogenin mRNA

1979 • 81 citations

Influenza antigenic determinants are expressed from haemagglutinin genes cloned in Escherichia coli

1980 • 78 citations

The use of R-looping for structural gene identification and mRNA purification

1979 • 75 citations

Stepwise biosynthesis in vitro of globin genes from globin mRNA by DNA polymerase of avian myeloblastosis virus.

1976 • 72 citations

Synthesis of an ovalbumin-like protein by Escherichia coli K12 harbouring a recombinant plasmid

1978 • 71 citations

Cloning of an almost full-length chicken conalbumin double-stranded cDNA

1979 • 70 citations

Synthesis of growth hormone by bacteria

1978 • 68 citations

Isolation and partial sequence of recombinant plasmids containing human α-, β- and γ-globin cDNA fragments

1978 • 67 citations

Cell-Free Translation of Messenger RNA of Simian Virus 40: Synthesis of the Major Capsid Protein

1974 • 65 citations

Synthesis of simian virus 40 t antigen in Escherichia coli.

1979 • 65 citations

The Initial Step of in Vitro Synthesis of Deoxyribonucleic Acid by the T4 Deoxyribonucleic Acid Polymerase

1971 • 64 citations

Immunoprecipitation of specific polysomes using Staphylococcus aureus: purification of the immunoglobulin .kappa. chain messenger RNA from the mouse myeloma MPC11

1978 • 61 citations

The ovalbumin gene. In vitro enzymatic synthesis and characterization.

1976 • 59 citations

Analysis of Xenopus laevis globins during development and erythroid cell maturation and the construction of recombinant plasmids containing sequences derived from adult globin mRNA

1979 • 52 citations

Cloning of DNA complementary to the measles virus mRNA encoding nucleocapsid protein.

1980 • 52 citations

Recombinant plasmids containing Xenopus laevis globin structural genes derived from complementary DNA

1978 • 49 citations

Integration of synthetic globin genes into an E. coli plasmid

1976 • 46 citations

A rapid method for the isolation of circular DNA using an aqueous two-phase partition system

1978 • 45 citations

Cloning and amplification of rabbit alpha- and beta-globin gene sequences into Escherichia coli plasmids.

1977 • 45 citations

Enzymic synthesis of deoxyribonucleic acid by the avian retrovirus reverse transcriptase in vitro: optimum conditions required for transcription of large ribonucleic acid templates

1980 • 41 citations

The ovalbumin gene. Insertion of ovalbumin gene sequences in chimeric bacterial plasmids.

1977 • 41 citations

Biological Sciences: Translation of Collagen mRNA from Chick Embryo Calvaria in a Cell-free System derived from Krebs II Ascites Cells

1973 • 38 citations

Loss of Simian Virus 40 DNA-RNA Hybrids from Nitrocellulose Membranes; Implications for the Study of Virus-Host DNA Interactions

1972 • 37 citations

Initiation of protein synthesis in bacteria at a translational start codon of mamalian cDNA: effects of the preceding nucleotide sequence.

1980 • 30 citations

RNA-DNA hybridization in solution without DNA reannealing

1977 • 30 citations

Isolation and Characterization of a Protein That Stimulates DNA Synthesis from Avian Myeloblastosis Virus

1972 • 29 citations

Polyadenylation and reverse transcription of influenza viral RNA

1979 • 29 citations

Synthesis of DNAs complementary to human ribosomal RNAs polyadenylated in vitro

1976 • 27 citations

Characterization of the estrogen-responsive domain of avian liver and cloning of double-stranded cDNA derived from estrogen-inducible RNA species.

1979 • 26 citations

Isolation of recombinant plasmids bearing cDNA to hen ovomucoid and lysozyme mRNAs.

1979 • 14 citations