SAM2 encodes the second methionine S-adenosyl transferase in Saccharomyces cerevisiae: physiology and regulation of both enzymes.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

1980 • 8,711 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

[2] New M13 vectors for cloning

1983 • 5,402 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA

1972 • 2,842 citations

Rapid and efficient cosmid cloning

1981 • 1,897 citations

Codon selection in yeast.

1982 • 1,580 citations

A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector

1987 • 1,181 citations

The Enzymic Synthesis of l-Cysteine in Escherichia coli and Salmonella typhimurium

1966 • 434 citations

Structure and function of the yeast URA3 gene: expression in Escherichia coli

1984 • 311 citations

The primary structures of two yeast enolase genes. Homology between the 5' noncoding flanking regions of yeast enolase and glyceraldehyde-3-phosphate dehydrogenase genes.

1981 • 215 citations

Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts

1980 • 200 citations

Methionine Adenosyltransferase ( S ‐Adenosylmethionine Synthetase) and S ‐Adenosylmethionine Decarboxylase

1984 • 177 citations

[5] Kilo-sequencing: Creation of an ordered nest of asymmetric deletions across a large target sequence carried on phage M13

1983 • 169 citations

Targeted deletion of a yeast enolase structural gene. Identification and isolation of yeast enolase isozymes.

1982 • 166 citations

Plasmids pEMBLY: new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences

1985 • 157 citations

An improved DNA sequencing strategy

1985 • 148 citations

Isolation and DNA Sequence of ADH3, a Nuclear Gene Encoding the Mitochondrial Isozyme of Alcohol Dehydrogenase in Saccharomyces cerevisiae

1985 • 146 citations

Mitochondrial and Nonmitochondrial Citrate Synthases in Saccharomyces cerevisiae Are Encoded by Distinct Homologous Genes

1986 • 109 citations

The sequence of metK, the structural gene for S-adenosylmethionine synthetase in Escherichia coli.

1984 • 108 citations

Activation of methionine for transmethylation. Purification of the S-adenosylmethionine synthetase of bakers' yeast and its separation into two forms.

1977 • 103 citations

Acyl Derivatives of Homoserine as Substrates for Homocysteine Synthesis in Neurospora crassa, Yeast, and Escherichia coli

1967 • 96 citations

Properties of metK Mutants of Escherichia coli K-12

1973 • 96 citations

SAM1, the structural gene for one of the S-adenosylmethionine synthetases in Saccharomyces cerevisiae. Sequence and expression.

1987 • 90 citations

Transcriptional regulation of the MET3 gene of Saccharomyces cerevisiae

1985 • 85 citations

Differential regulation of the duplicated isocytochrome c genes in yeast.

1984 • 82 citations

The Regulation of Isoleucine‐Valine Biosynthesis in Saccharomyces cerevisiae

1968 • 76 citations

The Saccharomyces cerevisiae MET3 gene: Nucleotide sequence and relationship of the 5′ non-coding region to that of MET25

1987 • 74 citations

Nucleotide sequence of theSaccharomyces cerevisiaeMET25 gene

1986 • 74 citations

S-adenosyl methionine requiring mutants in Saccharomyces cerevisiae: Evidences for the existence of two methionine adenosyl transferases

1978 • 70 citations

Intracellular sorting of alcohol dehydrogenase isoenzymes in yeast: a cytosolic location reflects absence of an amino-terminal targeting sequence for the mitochondrion.

1986 • 63 citations

Effects of Regulatory Mutations upon Methionine Biosynthesis in Saccharomyces cerevisiae: Loci eth2-eth3-eth10

1973 • 62 citations

[17] Cloning of the Escherichia coli genes for the biosynthetic enzymes for polyamines

1983 • 27 citations