MBP Fusion Protein with a Viral Protease Cleavage Site: One-Step Cleavage/Purification of Insoluble Proteins

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

Metal chelate affinity chromatography, a new approach to protein fractionation

1975 • 2,050 citations

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

1987 • 1,107 citations

Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli

1995 • 733 citations

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

1988 • 662 citations

An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein

1988 • 539 citations

[29] Synthesis and sequence-specific proteolysis of hybrid proteins produced in Escherichia coli

1987 • 392 citations

Commonality of the gene programs induced by effectors of apoptosis in androgen-dependent and -independent prostate cells.

1994 • 229 citations

Expression and Function of the Leucine Zipper Protein Par-4 in Apoptosis

1997 • 205 citations

Substrate requirements of human rhinovirus 3C protease for peptide cleavage in vitro.

1990 • 203 citations

Efficient and Rapid Affinity Purification of Proteins Using Recombinant Fusion Proteases

1994 • 157 citations

Phosphorylation of recombinant human phenylalanine hydroxylase: effect on catalytic activity, substrate activation and protection against non-specific cleavage of the fusion protein by restriction protease

1996 • 60 citations

Sensitive Ribonuclease Protection Assay Employing Glycogen as a Carrier and a Single Inactivation/Precipitation Step

2001 • 21 citations

Expression of human tyrosine hydroxylase type I in Escherichia coli as a protease-cleavable fusion protein

1999 • 10 citations