Cloning of Amb a I (antigen E), the major allergen family of short ragweed pollen.

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

1979 • 22,482 citations

A comprehensive set of sequence analysis programs for the VAX

1984 • 14,824 citations

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

1977 • 12,015 citations

Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

1985 • 9,128 citations

Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

1987 • 5,066 citations

Screening λgt Recombinant Clones by Hybridization to Single Plaques in Situ

1977 • 4,696 citations

A new method for predicting signal sequence cleavage sites

1986 • 4,629 citations

A simple and very efficient method for generating cDNA libraries

1983 • 4,606 citations

Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs

1984 • 3,334 citations

Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes

1981 • 1,265 citations

T-cell antigenic sites tend to be amphipathic structures.

1985 • 527 citations

Molecular cloning of complementary DNA encoding the lignin-forming peroxidase from tobacco: Molecular analysis and tissue-specific expression

1987 • 422 citations

Isolation and Characterization of Allergens from Ragweed Pollen. II^*

1964 • 270 citations

The Subclass Nature and Clinical Significance of the IgG Antibody Response in Patients Undergoing Allergen‐Specific Immunotherapy

1985 • 210 citations

Characterization of a pollen-specific cDNA clone from Zea mays and its expression.

1989 • 190 citations

Immunotherapy of hay fever with ragweed antigen E: Comparisons with whole pollen extract and placebos

1968 • 187 citations

cDNA cloning and primary structure of a white-face hornet venom allergen, antigen 5.

1988 • 157 citations

Analysis of the cDNA for phospholipase A₂ from honeybee venom glands

1989 • 153 citations

Use of o-phthalaldehyde to reduce background during automated Edman degradation

1984 • 138 citations

Chemical and Biological Properties of Some Atopic Allergens

1976 • 137 citations

A procedure for in situ alkylation of cystine residues on glass fiber prior to protein microsequence analysis

1987 • 129 citations

Cloning and Expression of DNA Coding for the Major House Dust Mite Allergen <i>Der p</i> 1 in <i>Escherichia coli</i>

1988 • 119 citations

Immunogenic properties of modified antigen E. II. Ability of urea-denatured antigen and alpha-polypeptide chain to prime T cells specific for antigen E.

1975 • 92 citations

A Solid Phase Radioimmunoassay for the Quantitation of Human Reaginic Antibody against Ragweed Antigen E

1973 • 92 citations

Further structural studies of the heavy chain of HLA antigens and its similarity to immunoglobulins.

1977 • 75 citations

A double-blind, placebo-controlled trial of polymerized whole ragweed for immunotherapy of ragweed allergy

1982 • 75 citations

Immunogenic Properties of Modified Antigen E

1975 • 68 citations

Immunogenic properties of modified antigen E. I. Presence of specific determinants for T cells in denatured antigen and polypeptide chains.

1974 • 58 citations

Quantitative relationships between IgE antibody and blocking antibodies specific for antigen E in patients given immunotherapy with ragweed antigen E.

1977 • 55 citations

Two major human allergenic sites on ragweed pollen allergen antigen E identified by using monoclonal antibodies.

1986 • 51 citations

Tracking Down Plant Genes: Paths, Patterns, and Footprints

1989 • 46 citations

A strategy for single site PCR amplification of dsDNA: priming digested cloned or genomic DNA from an anchor-modified restriction site and a short internal sequence.

1990 • 45 citations

Computer programs for handling nucleic acid sequences

1984 • 38 citations

A rapid method for cloning and sequencing variable-region genes of expressed immunoglobulins

1987 • 38 citations

Immunogenic Properties of Modified Antigen E

1974 • 36 citations

Antigens of Ambrosia elatior (short ragweed) pollen. III. Crossed radioimmunoelectrophoresis of ragweed-allergic patients' sera with special attention to quantification of IgE responses.

1983 • 29 citations

Are plant genes different?

1983 • 25 citations

Limited proteolysis of antigens E and K from ragweed pollen

1981 • 22 citations

Immunologic responses to conjugates of antigen E in patients with ragweed hay fever

1984 • 20 citations

Immunotherapy with short ragweed fraction A: -glutamic acid: -lysine polymer in ragweed hay fever

1981 • 13 citations

Monoclonal antibodies to denatured ragweed pollen allergen Amb a I: characterization, specificity for the denatured allergen, and utilization for the isolation of immunogenic peptides of Amb a I

1988 • 11 citations