Novel purification of the catalytic domain of Golgi alpha-mannosidase II. Characterization and comparison with the intact enzyme.

Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

1987 • 5,066 citations

ASSEMBLY OF ASPARAGINE-LINKED OLIGOSACCHARIDES

1985 • 4,790 citations

Phase separation of integral membrane proteins in Triton X-114 solution.

1981 • 3,366 citations

Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: Evidence for membrane cycling from Golgi to ER

1989 • 1,685 citations

Microtubule-dependent retrograde transport of proteins into the ER in the presence of brefeldin a suggests an ER recycling pathway

1990 • 972 citations

Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum.

1990 • 918 citations

Glycosyltransferases

1989 • 904 citations

Progress in Unraveling Pathways of Golgi Traffic

1985 • 615 citations

Short cytoplasmic sequences serve as retention signals for transmembrane proteins in the endoplasmic reticulum

1989 • 499 citations

Primary structure of beta-galactoside alpha 2,6-sialyltransferase. Conversion of membrane-bound enzyme to soluble forms by cleavage of the NH2-terminal signal anchor.

1987 • 408 citations

Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action.

1990 • 384 citations

Cloning and characterization of DNA complementary to human UDP-GalNAc: Fuc alpha 1----2Gal alpha 1----3GalNAc transferase (histo-blood group A transferase) mRNA.

1990 • 360 citations

Rapid visualization of protein bands in preparative SDS-polyacrylamide gels

1979 • 310 citations

alpha-D-Mannosidases of rat liver Golgi membranes. Mannosidase II is the GlcNAcMAN5-cleaving enzyme in glycoprotein biosynthesis and mannosidases Ia and IB are the enzymes converting Man9 precursors to Man5 intermediates.

1982 • 294 citations

Agarose Derivatives of Uridine Diphosphate and N-Acetylglucosamine for the Purification of a Galactosyltransferase

1972 • 290 citations

A monoclonal antibody against a 135-K Golgi membrane protein.

1982 • 262 citations

Purification of a Gal beta 1 to 4GlcNAc alpha 2 to 6 sialyltransferase and a Gal beta 1 to 3(4)GlcNAc alpha 2 to 3 sialyltransferase to homogeneity from rat liver.

1982 • 248 citations

Isolation of a Golgi-rich fraction from rat liver

1970 • 222 citations

A short sequence in the COOH-terminus makes an adenovirus membrane glycoprotein a resident of the endoplasmic reticulum

1987 • 202 citations

Purification and characterization of alpha-D-mannosidase from rat liver golgi membranes.

1977 • 178 citations

Biosynthesis of terminal Gal alpha 1—-3Gal beta 1—-4GlcNAc-R oligosaccharide sequences on glycoconjugates. Purification and acceptor specificity of a UDP-Gal:N-acetyllactosaminide alpha 1—-3-galactosyltransferase from calf thymus.

1985 • 174 citations

Protein Transfer and Organelle Biogenesis

1988 • 146 citations

Control of glycoprotein synthesis. Purification and characterization of rabbit liver UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I.

1988 • 130 citations

Conversion of a Golgi apparatus sialyltransferase to a secretory protein by replacement of the NH2-terminal signal anchor with a signal peptide

1989 • 126 citations

Isolation to homogeneity and partial characterization of a histo-blood group A defined Fuc alpha 1----2Gal alpha 1----3-N-acetylgalactosaminyltransferase from human lung tissue.

1990 • 124 citations

Signals for retention of transmembrane proteins in the endoplasmic reticulum studied with CD4 truncation mutants.

1991 • 122 citations

The E1 glycoprotein of an avian coronavirus is targeted to the cis Golgi complex.

1990 • 118 citations

Control of glycoprotein synthesis. Purification of UDP-N-acetylglucosamine:alpha-D-mannoside beta 1-2 N-acetylglucosaminyltransferase II from rat liver.

1987 • 96 citations

Biosynthesis and modification of Golgi mannosidase II in HeLa and 3T3 cells.

1985 • 94 citations

Immunocytochemical localization of alpha-D-mannosidase II in the Golgi apparatus of rat liver.

1983 • 93 citations

Glycoprotein biosynthesis in Saccharomyces cerevisiae. Purification of the alpha-mannosidase which removes one specific mannose residue from Man9GlcNAc.

1988 • 87 citations

Purification and enzymatic characterization of CMP-sialic acid: beta-galactosyl1----3-N-acetylgalactosaminide alpha 2----3-sialyltransferase from human placenta.

1985 • 74 citations

The purification and characterization of mannosidase IA from rat liver Golgi membranes.

1988 • 72 citations

Characterization of trimming Man9-mannosidase from pig liver. Purification of a catalytically active fragment and evidence for the transmembrane nature of the intact 65 kDa enzyme

1989 • 63 citations

Isolation of a rat liver Golgi mannosidase II clone by mixed oligonucleotide-primed amplification of cDNA.

1989 • 51 citations

Purification and characterization of an α-1,2-mannosidase involved in processing asparagine-linked oligosaccharides

1989 • 47 citations

Mannosidase II and the 135-kDa Golgi-specific antigen recognized monoclonal antibody 53FC3 are the same dimeric protein.

1990 • 46 citations

Topology of mannosidase II in rat liver Golgi membranes and release of the catalytic domain by selective proteolysis.

1986 • 43 citations

The endoplasmic reticulum retention signal of the E3/19K protein of adenovirus-2 is microtubule binding

1991 • 35 citations

Purification and characterization of beta-galactoside (alpha 2 leads to 6)sialyltransferase from rat liver and hepatomas.

1982 • 30 citations

Purification and Characterization of β‐Galactoside (α2→6)sialyltransferase from Rat Liver and Hepatomas

1982 • 22 citations

Separation of peptides by reverse-phase high-performance liquid chromatography using propyl- and cyanopropylsilyl supports

1984 • 21 citations