N-(2-Aminoethyl)-5-isoquinolinesulfonamide, a newly synthesized protein kinase inhibitor, functions as a ligand in affinity chromatography. Purification of Ca2+-activated, phospholipid-dependent and other protein kinases.

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide-dependent protein kinase and protein kinase C

1984 • 2,728 citations

Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids.

1979 • 1,139 citations

Calcium-activated, phospholipid-dependent protein kinase from rat brain. Subcellular distribution, purification, and properties.

1982 • 1,008 citations

Studies on a cyclic nucleotide-independent protein kinase and its proenzyme in mammalian tissues. II. Proenzyme and its activation by calcium-dependent protease from rat brain.

1977 • 865 citations

Relationships between calcium and cyclic nucleotides in cell activation.

1977 • 849 citations

Calcium-dependent protein kinase: widespread occurrence in various tissues and phyla of the animal kingdom and comparison of effects of phospholipid, calmodulin, and trifluoperazine.

1980 • 568 citations

Binding of trifluoperazine to the calcium-dependent activator of cyclic nucleotide phosphodiesterase.

1977 • 544 citations

Calcium-induced exposure of a hydrophobic surface on calmodulin

1980 • 520 citations

Affinity Chromatography

1971 • 504 citations

[43] Preparation of homogeneous cyclic AMP-dependent protein kinase(s) and its subunits from rabbit skeletal muscle

1974 • 496 citations

Hydrophobic regions function in calmodulin-enzyme(s) interactions.

1980 • 363 citations

Phospholipid-sensitive calcium-dependent protein kinase: Inhibition by antipsychotic drugs

1981 • 243 citations

Phospholipid-sensitive Ca2+-dependent protein kinase from heart. I. Purification and general properties.

1982 • 235 citations

General ligands in affinity chromatography. Cofactor–substrate elution of enzymes bound to the immobilized nucleotides adenosine 5′-monophosphate and nicotinamide–adenine dinucleotide

1972 • 202 citations

Widespread Occurence of Calcium-Activated, Phospholipid Dependent Protein Kinase in Mammalian Tissues1

1981 • 196 citations

Composition of the myosin light chain kinase from chicken gizzard

1977 • 192 citations

A novel vascular relaxing agent, N-(6--aminohexyl)-5-chloro-1-naphthalensulfonamide which affects vascular smooth muscle actomyosin.

1978 • 171 citations

Protein binding by agarose carrying hydrophobic groups in conjunction with charges

1973 • 163 citations

Purified rat brain calcium- and phospholipid-dependent protein kinase phosphorylates ribosomal protein S6.

1983 • 160 citations

Two types of calcium-dependent protein phosphorylations modulated by calmodulin antagonists. Naphthalenesulfonamide derivatives.

1982 • 156 citations

Purification to homogeneity, characterization and monoclonal antibodies of phospholipid-sensitive Ca2+-dependent protein kinase from spleen

1983 • 94 citations

Most of the Ca2+-dependent endogenous phosphorylation of rat brain cytosol proteins requires Ca2+-dependent regulator protein

1979 • 51 citations

Affinity chromatography and insoluble enzymes

1971 • 29 citations

Cyclic AMP-dependent protein kinase: Purification of the holoenzyme by affinity chromatography

1976 • 25 citations

General Ligand Affinity Chromatography in Enzyme Purification Ligands, Affinity Chromatography, Enzyme Purification

1976 • 22 citations

Affinity chromatography of biological macromolecules

1971 • 21 citations

Calcium and cell function Vol. IV

1985 • 20 citations

Hydrophobic properties of Tetrahymena calmodulin related to the phosphodiesterase activity

1983 • 14 citations

[47] Purification and characterization of cyclic GMP-dependent protein kinases

1974 • 12 citations

Application of general ligand affinity chromatography for the mutual separation of deoxyribonuclease and ribonuclease free of protease contamination

1976 • 12 citations