Cyclic AMP responses are suppressed in mammalian cells expressing the yeast low Km cAMP-phosphodiesterase gene.

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.

1982 • 8,069 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1984 • 4,899 citations

A highly sensitive adenylate cyclase assay

1974 • 4,213 citations

CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements

1987 • 1,792 citations

Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae.

1986 • 295 citations

Effect of Forskolin on Voltage-Gated K ⁺ Channels Is Independent of Adenylate Cyclase Activation

1988 • 269 citations

Molecular analysis of cDNA clones and the corresponding genomic coding sequences of the Drosophila dunce+ gene, the structural gene for cAMP phosphodiesterase.

1986 • 253 citations

Inhibition of intracellular cAMP-dependent protein kinase using mutant genes of the regulatory type I subunit.

1987 • 248 citations

Rigorous feedback control of cAMP levels in Saccharomyces cerevisiae.

1987 • 230 citations

A Protein Kinase Inhibitor Gene Reduces Both Basal and Multihormone-stimulated Prolactin Gene Transcription

1989 • 203 citations

Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase.

1989 • 192 citations

Modulation of Acetylcholine Receptor Desensitization by Forskolin Is Independent of cAMP

1988 • 154 citations

Molecular cloning and developmental expression of the cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum.

1986 • 151 citations

Cloning and characterization of mammalian homologs of the Drosophila dunce+ gene.

1989 • 147 citations

Recombinant Fragment of Protein Kinase Inhibitor Blocks Cyclic AMP-Dependent Gene Transcription

1987 • 139 citations

Isolation and characterization of the rat tyrosine aminotransferase gene.

1984 • 127 citations

Transcriptional activation of the rat liver tyrosine aminotransferase gene by cAMP.

1984 • 125 citations

Isolation of multiple classes of mutants of CHO cells resistant to cyclic AMP

1980 • 92 citations

Transformation of Chick-Embryo Fibroblasts by Wild-Type and Temperature-Sensitive Rous Sarcoma Virus Alters Adenylate Cyclase Activity

1973 • 75 citations

Soft and Hard Tissue Repair: Biological and Clinical Aspects

1984 • 74 citations

SRA5 Encodes the Low-K_m Cyclic AMP Phosphodiesterase of Saccharomyces cerevisiae

1988 • 71 citations

Efficient and stable expression of recombinant fibronectin polypeptides.

1987 • 55 citations

Sequences Upstream from the Mousec-mosOncogene May Function as a Transcription Termination Signal

1986 • 54 citations

A mutation affecting the catalytic subunit of cyclic AMP-dependent protein kinase in CHO cells.

1979 • 46 citations

Purification of intact and nicked forms of a zinc-containing, Mg2+-dependent, low Km cyclic AMP phosphodiesterase from bakers' yeast.

1984 • 45 citations

Transfer of genes to chinese hamster ovary cells by DNA-mediated transformation

1982 • 36 citations

Characterization of a cyclic AMP-resistant Chinese hamster ovary cell mutant containing both wild-type and mutant species of type I regulatory subunit of cyclic AMP-dependent protein kinase.

1985 • 33 citations

Characterization of an adenosine 3′:5′-cyclic monophosphate phosphodiesterase from baker's yeast. Its binding to subcellular particles, catalytic properties and gel-filtration behaviour

1977 • 27 citations

Isolation and partial characterization of a cyclic GMP-dependent cyclic GMP-specific phosphodiesterase from Dictyostelium discoideum

1983 • 22 citations

Characterization of the yeast low Km cAMP-phosphodiesterase with cAMP analogues. Applications in mammalian cells that express the yeast PDE2 gene.

1990 • 21 citations

Agents for the treatment of heart failure

1983 • 17 citations

DNA‐mediated transfer of cAMP resistance in CHO cells

1986 • 3 citations