Gene Splicing by Overlap Extension: Tailor-Made Genes Using the Polymerase Chain Reaction

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

1987 • 5,745 citations

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction

1986 • 3,400 citations

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

1989 • 3,067 citations

A general method ofin vitropreparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions

1988 • 2,410 citations

The "megaprimer" method of site-directed mutagenesis.

1990 • 1,174 citations

Using mini-prep plasmid DNA for sequencing double stranded templates with Sequenase.

1988 • 552 citations

Ancient DNA and the polymerase chain reaction

1989 • 461 citations

Precise gene fusion by PCR

1989 • 292 citations

Regions of allelic hypervariability in the murine Aα immune response gene

1983 • 185 citations

Use of polymerase chain reaction catalyzed by Taq DNA polymerase for site-specific mutagenesis

1989 • 127 citations

USE OF SYNTHETIC OLIGODEOXYRIBONUCLEOTIDES FOR THE ISOLATION OF SPECIFIC CLONED DNA SEQUENCES11Supported by NIH Postdoctoral Fellowship GM07591 (SVS) and NIH grants GM26391 (RBW) and GM 25658 (KI).

1981 • 106 citations

Sequence analysis and structure-function correlations of murine q, k, u, s, and f haplotype I-A beta cDNA clones.

1986 • 105 citations

The functional significance of two amino acid polymorphisms in the antigen-presenting domain of class I MHC molecules. Molecular dissection of Kbm3.

1989 • 36 citations

Directed mutagenesis using PCR

1989 • 33 citations