Mechanism of guanine nucleotide regulatory protein-mediated inhibition of adenylate cyclase. Studies with isolated subunits of transducin in a reconstituted system.

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

G proteins and dual control of adenylate cyclase

1984 • 1,811 citations

The role of hormone receptors and GTP-regulatory proteins in membrane transduction

1980 • 1,750 citations

Identification of the predominant substrate for ADP-ribosylation by islet activating protein.

1983 • 583 citations

The regulatory component of adenylate cyclase. Purification and properties.

1981 • 505 citations

Purification and properties of the inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase.

1984 • 498 citations

Purification of the regulatory component of adenylate cyclase.

1980 • 497 citations

Characterization of transducin from bovine retinal rod outer segments. I. Separation and reconstitution of the subunits.

1983 • 492 citations

The inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase. Properties and function of the purified protein.

1984 • 411 citations

The subunits of the stimulatory regulatory component of adenylate cyclase. Resolution of the activated 45,000-dalton (alpha) subunit.

1983 • 380 citations

The inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase. Subunit dissociation and guanine nucleotide-dependent hormonal inhibition.

1984 • 345 citations

Stimulation and inhibition of adenylyl cyclases mediated by distinct regulatory proteins

1983 • 309 citations

The regulatory components of adenylate cyclase and transducin. A family of structurally homologous guanine nucleotide-binding proteins.

1983 • 304 citations

Resolution of some components of adenylate cyclase necessary for catalytic activity.

1977 • 283 citations

The inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase. Subunit dissociation and the inhibition of adenylate cyclase in S49 lymphoma cyc- and wild type membranes.

1984 • 265 citations

Mammalian .beta.2-adrenergic receptor: reconstitution of functional interactions between pure receptor and pure stimulatory nucleotide binding protein of the adenylate cyclase system

1984 • 249 citations

Ns and Ni, the stimulatory and inhibitory regulatory components of adenylyl cyclases. Purification of the human erythrocyte proteins without the use of activating regulatory ligands.

1984 • 242 citations

The subunits of the stimulatory regulatory component of adenylate cyclase. Resolution, activity, and properties of the 35,000-dalton (beta) subunit.

1983 • 237 citations

Pertussis toxin substrate, the putative Ni component of adenylyl cyclases, is an alpha beta heterodimer regulated by guanine nucleotide and magnesium.

1983 • 237 citations

Identification of a gamma subunit associated with the adenylyl cyclase regulatory proteins Ns and Ni.

1984 • 214 citations

Specificity of the functional interactions of the beta-adrenergic receptor and rhodopsin with guanine nucleotide regulatory proteins reconstituted in phospholipid vesicles.

1985 • 213 citations

Molecular Cloning of Complementary DNA for the Alpha Subunit of the G Protein that Stimulates Adenylate Cyclase

1985 • 209 citations

Mammalian .beta.2-adrenergic receptor: purification and characterization

1984 • 207 citations

A nucleotide regulatory site for somatostatin inhibition of adenylate cyclase in S49 lymphoma cells

1983 • 200 citations

Membrane-dependent guanine nucleotide binding and GTPase activities of soluble protein from bovine rod cell outer segments.

1979 • 183 citations

Use of specific antibodies to quantitate the guanine nucleotide-binding protein Go in brain.

1986 • 158 citations

[23] Purification of rhodopsin by concanavalin A affinity chromatography

1982 • 157 citations

Guanine nucleotide inhibition of cyc- S49 mouse lymphoma cell membrane adenylyl cyclase.

1982 • 151 citations

Chapter 5 First Stage of Amplification in the Cyclic-Nucleotide Cascade of Vision

1981 • 121 citations

Reconstitution of a hormone-sensitive adenylate cyclase system. The pure beta-adrenergic receptor and guanine nucleotide regulatory protein confer hormone responsiveness on the resolved catalytic unit.

1984 • 120 citations

The beta 1-adrenergic receptor of the turkey erythrocyte. Molecular heterogeneity revealed by purification and photoaffinity labeling.

1982 • 117 citations

Characterization by two-dimensional peptide mapping of the gamma subunits of Ns and Ni, the regulatory proteins of adenylyl cyclase, and of transducin, the guanine nucleotide-binding protein of rod outer segments of the eye.

1985 • 114 citations

Properties of the separated catalytic and regulatory units of brain adenylate cyclase.

1980 • 111 citations

Effects of guanyl nucleotides and rhodopsin on ADP-ribosylation of the inhibitory GTP-binding component of adenylate cyclase by pertussis toxin.

1984 • 100 citations

A role for Ni in the hormonal stimulation of adenylate cyclase

1985 • 99 citations

Reconstitution of cholera toxin-activated adenylate cyclase.

1978 • 98 citations

Antibodies against the carboxyl-terminal 5-kDa peptide of the alpha subunit of transducin crossreact with the 40-kDa but not the 39-kDa guanine nucleotide binding protein from brain.

1985 • 98 citations

The beta-adrenergic receptor: rapid purification and covalent labeling by photoaffinity crosslinking.

1982 • 76 citations

Organization of rhodopsin in photoreceptor membranes. 1. Proteolysis of bovine rhodopsin in native membranes and the distribution of sulfhydryl groups in the fragments

1978 • 65 citations

Inhibitory regulation of adenylyl cyclase in the absence of stimulatory regulation. Requirements and kinetics of guanine nucleotide-induced inhibition of the cyc- S49 adenylyl cyclase.

1983 • 65 citations

Interaction of the stimulatory and inhibitory regulatory proteins of the adenylyl cyclase system with the catalytic component of cyc-S49 cell membranes.

1984 • 52 citations

Antibodies against a retinal guanine nucleotide‐binding protein cross‐react with a single plasma membrane protein in non‐retinal tissues

1984 • 51 citations

Transducin and the inhibitory nucleotide regulatory protein inhibit the stimulatory nucleotide regulatory protein mediated stimulation of adenylate cyclase in phospholipid vesicle systems

1985 • 43 citations