Surfactant protein A amino acids Glu195 and Arg197 are essential for receptor binding, phospholipid aggregation, regulation of secretion, and the facilitated uptake of phospholipid by type II cells.

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Application to the radioimmunoassay

1973 • 3,279 citations

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

1989 • 3,067 citations

Structure of a C-type mannose-binding protein complexed with an oligosaccharide

1992 • 873 citations

Engineering galactose-binding activity into a C-type mannose-binding protein

1992 • 470 citations

Mannose-binding proteins isolated from rat liver contain carbohydrate-recognition domains linked to collagenous tails. Complete primary structures and homology with pulmonary surfactant apoprotein.

1986 • 433 citations

Isolation and characterization of the human pulmonary surfactant apoprotein gene

1985 • 397 citations

Nucleotide and amino acid sequences of pulmonary surfactant protein SP 18 and evidence for cooperation between SP 18 and SP 28-36 in surfactant lipid adsorption.

1987 • 382 citations

Pulmonary Alveolar Type II Cells Isolated from Rats

1979 • 344 citations

Reconstitution of Tubular Myelin from Synthetic Lipids and Proteins Associated with Pig Pulmonary Surfactant

1989 • 338 citations

Effects of a surfactant-associated protein and calcium ions on the structure and surface activity of lung surfactant lipids

1985 • 326 citations

Surfactant apoprotein Mr = 26,000-36,000 enhances uptake of liposomes by type II cells.

1987 • 309 citations

The major lung surfactant protein, SP 28-36, is a calcium-dependent, carbohydrate-binding protein.

1987 • 281 citations

Characterization studies on a new lectin found in seeds of Vicia ervilia

1975 • 275 citations

Pulmonary surfactant and its components inhibit secretion of phosphatidylcholine from cultured rat alveolar type II cells.

1987 • 242 citations

Surfactant-associated protein inhibits phospholipid secretion from type II cells

1987 • 200 citations

Alveolar type II cells express a high-affinity receptor for pulmonary surfactant protein A.

1988 • 190 citations

Pulmonary surfactant protein A (SP-A) specifically binds dipalmitoylphosphatidylcholine.

1991 • 156 citations

Pulmonary surfactant apoprotein A structure and modulation of surfactant secretion by rat alveolar type II cells.

1988 • 135 citations

Phospholipid binding and biophysical activity of pulmonary surfactant-associated protein (SAP)-35 and its non-collagenous COOH-terminal domains.

1986 • 125 citations

Lung surfactant apoprotein SP-A (26-36 kDa) binds with high affinity to isolated alveolar type II cells.

1989 • 112 citations

Isolation and sequence of a cDNA clone for the rat pulmonary surfactant-associated protein (PSP-A)

1987 • 112 citations

Binding and uptake of pulmonary surfactant protein (SP-A) by pulmonary type II epithelial cells.

1989 • 99 citations

Chimeras of surfactant proteins A and D identify the carbohydrate recognition domains as essential for phospholipid interaction.

1994 • 84 citations

Chemical modification of surfactant protein A alters high affinity binding to rat alveolar type II cells and regulation of phospholipid secretion.

1988 • 76 citations

Alveolar Type II Cells, Surfactant Protein A (SP-A), and the Phospholipid Components of Surfactant in Acute Silicosis in the Rat

1989 • 74 citations

The structure and function of surfactant protein A. Hydroxyproline- and carbohydrate-deficient mutant proteins.

1994 • 63 citations

Epitope mapping for monoclonal antibodies identifies functional domains of pulmonary surfactant protein A that interact with lipids.

1994 • 59 citations

Regulation of surfactant phospholipid secretion from isolated rat alveolar type II cells by lectins

1988 • 22 citations

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