Messenger RNA synthesis in mammalian cells is catalyzed by the phosphorylated form of RNA polymerase II.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycoluril

1978 • 4,673 citations

Silver staining of proteins in polyacrylamide gels

1981 • 3,565 citations

[42] Detection and quantification of phosphotyrosine in proteins

1983 • 1,184 citations

[17] Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis

1983 • 1,115 citations

Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.

1983 • 1,075 citations

Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.

1981 • 815 citations

Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases

1985 • 670 citations

Eukaryotic RNA Polymerase

1985 • 500 citations

Prokaryotic and eukaryotic RNA polymerases have homologous core subunits.

1987 • 355 citations

A unique structure at the carboxyl terminus of the largest subunit of eukaryotic RNA polymerase II.

1985 • 346 citations

[10] 5′-32P labeling of RNA and DNA restriction fragments

1980 • 251 citations

Purification using polyethylenimine precipitation and low molecular weight subunit analyses of calf thymus and wheat germ DNA-dependent RNA polymerase II

1977 • 222 citations

Phosphorylation of eukaryotic DNA-dependent RNA polymerase. Identification of calf thymus RNA polymerase subunits phosphorylated by two purified protein kinases, correlation with in vivo sites of phosphorylation in HeLa cell RNA polymerase II.

1981 • 159 citations

Purification and properties of calf thymus casein kinases I and II.

1981 • 157 citations

Animal DNA-Dependent RNA Polymerases. Molecular Structures and Immunological Properties of Calf-Thymus Enzyme AI and of Calf-Thymus and Rat-Liver Enzymes B

1974 • 143 citations

RNA contacts subunits IIo and IIc in HeLa RNA polymerase II transcription complexes.

1986 • 126 citations

Decomposition of phosphoserine and phosphothreonine during acid hydrolysis

1976 • 118 citations

Two Forms of RNA Polymerase B in Yeast

1976 • 107 citations

Phosphorylation of yeast DNA-dependent RNA polymerases in vivo and in vitro. Isolation of enzymes and identification of phosphorylated subunits.

1977 • 95 citations

Immunochemical analysis of mammalian RNA polymerase II subspecies. Stability and relative in vivo concentration.

1986 • 89 citations

Transcription of adenovirus-2 major late promoter inhibited by monoclonal antibody directed against RNA polymerases IIO and IIA.

1983 • 80 citations

5 Eukaryotic RNA Polymerases

1982 • 75 citations

Identification, molecular cloning, and mutagenesis of Saccharomyces cerevisiae RNA polymerase genes.

1984 • 71 citations

Monoclonal antibody specific for calf thymus RNA polymerases IIO and IIA.

1981 • 65 citations

The presence of phosphorylated subunits in yeast RNA polymerases A and B

1976 • 49 citations

The gene encoding the large subunit of human RNA polymerase II.

1985 • 48 citations

In vitro proteolysis of a large subunit of Drosophila melanogaster RNA polymerase B

1976 • 48 citations

On the Phosphorylation of Yeast RNA Polymerases A and B

1983 • 42 citations

Identification of a structural gene for a RNA polymerase II polypeptide in Drosophila melanogaster and mammalian species.

1983 • 40 citations

Calf thymus RNA polymerases I and II do not contain subunits structurally related to casein kinases I and II.

1981 • 37 citations

A general procedure for the end labeling of proteins and positioning of amino acids in the sequence.

1984 • 31 citations

Size heterogeneity of the largest subunit of nuclear RNA polymerase II. An immunological analysis.

1984 • 28 citations

Structural relationship between the large subunits of calf thymus RNA polymerase II.

1983 • 24 citations