Structure-function studies of bacteriorhodopsin XV. Effects of deletions in loops B-C and E-F on bacteriorhodopsin chromophore and structure

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Studies on transformation of Escherichia coli with plasmids

1983 • 11,179 citations

Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy

1990 • 2,969 citations

Rapid and efficient cosmid cloning

1981 • 1,897 citations

Reconstitution of Purple Membrane Vesicles Catalyzing Light-driven Proton Uptake and Adenosine Triphosphate Formation

1974 • 714 citations

A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels

1979 • 460 citations

The structural basis of the functioning of bacteriorhodopsin: An overview

1979 • 459 citations

Amino acid sequence of bacteriorhodopsin.

1979 • 405 citations

Path of the polypeptide in bacteriorhodopsin.

1980 • 366 citations

Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication

1983 • 362 citations

Sequence of the gene for iso-l-cytochrome c in saccharomyces cerevisiae

1979 • 348 citations

Refolding of an integral membrane protein. Denaturation, renaturation, and reconstitution of intact bacteriorhodopsin and two proteolytic fragments.

1981 • 332 citations

Refolding of bacteriorhodopsin in lipid bilayers

1987 • 271 citations

Structural studies on transmembrane proteins. 2. Spin labeling of bacteriorhodopsin mutants at unique cysteines

1989 • 263 citations

Denaturation and renaturation of bacteriorhodopsin in detergents and lipid-detergent mixtures.

1982 • 243 citations

The bacteriorhodopsin gene.

1981 • 240 citations

Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit β-globin

1980 • 227 citations

Binding of all-trans-retinal to the purple membrane. Evidence for cooperativity and determination of the extinction coefficient

1979 • 170 citations

Specific amino acid substitutions in bacterioopsin: Replacement of a restriction fragment in the structural gene by synthetic DNA fragments containing altered codons

1984 • 158 citations

Light‐dependent reaction of bacteriorhodopsin with hydroxylamine in cell suspensions of Halobacterium halobium: Demonstration of an APO‐membrane

1974 • 155 citations

Site of attachment of retinal in bacteriorhodopsin.

1981 • 146 citations

Structure-function studies on bacteriorhodopsin. IV. Purification and renaturation of bacterio-opsin polypeptide expressed in Escherichia coli.

1987 • 123 citations

Orientation of retinal in bacteriorhodopsin as studied by cross-linking using a photosensitive analog of retinal.

1982 • 102 citations

Immunological probes for bacteriorhodopsin. Identification of three distinct antigenic sites on the cytoplasmic surface.

1982 • 50 citations

Structure-function studies on bacteriorhodopsin. II. Improved expression of the bacterio-opsin gene in Escherichia coli.

1987 • 47 citations

Removal of the carboxyl-terminal peptide does not affect refolding or function of bacteriorhodopsin as a light-dependent proton pump.

1984 • 45 citations

Topography of surface-exposed amino acids in the membrane protein bacteriorhodopsin determined by proteolysis and micro-sequencing

1989 • 31 citations

Introduction and characterization of amber mutations in the bacteriorhodopsin gene.

1983 • 13 citations