Characterization of 2',3'-O-(2,4,6-trinitrocyclohexadienylidine)adenosine 5'-triphosphate as a fluorescent probe of the ATP site of sodium and potassium transport adenosine triphosphatase. Determination of nucleotide binding stoichiometry and ion-induced changes in affinity for ATP.

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS

1949 • 19,979 citations

A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples

1978 • 6,494 citations

Activation by Adenosine Triphosphate in the Phosphorylation Kinetics of Sodium and Potassium Ion Transport Adenosine Triphosphatase

1972 • 826 citations

Determination of the absolute quantum yield of fluorescent solutions

1957 • 657 citations

A characterization of vanadate interactions with the (Na,K)-ATPase. Mechanistic and regulatory implications.

1978 • 520 citations

RESOLUTION OF THE FLUORESCENCE EXCITATION SPECTRUM OF INDOLE INTO THE ¹L_a AND ¹L_b EXCITATION BANDS*

1977 • 387 citations

Membrane enzyme systems molecular size determinations by radiation inactivation

1968 • 358 citations

Binding of Adenosine Triphosphate to Sodium and Potassium Ion-stimulated Adenosine Triphosphatase

1971 • 349 citations

Studies on the Characterization of the Sodium-Potassium Transport Adenosine Triphosphatase

1973 • 305 citations

[24] Nanosecond fluorescence spectroscopy of macromolecules

1972 • 294 citations

HIGHER DERIVATIVE ANALYSIS OF COMPLEX ABSORPTION SPECTRA

1970 • 290 citations

Characterization of a new photoaffinity derivative of ouabain: labeling of the large polypeptide and of a proteolipid component of the (sodium-potassium ion)-dependent ATPase

1978 • 282 citations

Anionic sigma complexes

1970 • 267 citations

Binding of ATP to brain microsomal ATPase Determination of the ATP-binding capacity and the dissociation constant of the enzyme-ATP complex as a function of K+ concentration

1971 • 263 citations

Influence of single amino acid substitutions on electrophoretic mobility of sodium dodecyl sulfate-protein complexes

1978 • 240 citations

Biochemistry of Membrane Transport

1977 • 235 citations

Tryptophan fluorescence of (Na+ + K+)-ATPase as a tool for study of the enzyme mechanism

1978 • 231 citations

Conformational transitions between Na+-bound and K+-bound forms of (Na+ + K+)-ATPase, studied with formycin nucleotides

1978 • 222 citations

Purification and characterization of (Na+ + K+)-ATPase IV. Estimation of the purity and of the molecular weight and polypeptide content per enzyme unit in preparations from the outer medulla of rabbit kidney

1974 • 211 citations

Purification and Characterization of and [3H]Ouabain Binding to the Transport Adenosine Triphosphatase from Outer Medulla of Canine Kidney

1973 • 210 citations

ATP hydrolysis associated with an uncoupled sodium flux through the sodium pump: evidence for allosteric effects of intracellular ATP and extracellular sodium

1976 • 204 citations

Measurement of inorganic orthophosphate in biological materials: Extraction properties of butyl acetate

1974 • 200 citations

Preparation and properties of 2′(or 3′)-O-(2,4,6-trinitrophenyl) adenosine 5′-triphosphate, an analog of adenosine triphosphate

1973 • 161 citations

The Effect of Delipidation on the Adenosine Triphosphatase of Sarcoplasmic Reticulum. Electron Microscopy and Physical Properties

1974 • 152 citations

Inhibition of sodium and potassium adenosine triphosphatase by 2‘,3‘-O-(2,4,6-trinitrocyclohexadienylidene) adenine nucleotides. Implications for the structure and mechanism of the Na:K pump.

1981 • 150 citations

Substrate sites of the (Na+ + K+)-dependent ATPase

1976 • 142 citations

Purification and characterization of (Na+ + K+)-ATPase. VI. Differential tryptic modification of catalytic functions of the purified enzyme in presence of NaCl and KCl

1977 • 133 citations

Stoichiometry and molecular weight of the minimum asymmetric unit of canine renal sodium and potassium ion-activated adenosine triphosphatase.

1980 • 125 citations

Potassium: potassium exchange catalysed by the sodium pump in human red cells

1974 • 120 citations

Occlusion of K ions in the unphosphorylated sodium pump

1979 • 119 citations

Molecular properties of purified (sodium + potassium)-activated adenosine triphosphatases and their subunits from the rectal gland of Squalus acanthias and the electric organ of Electrophorus electricus

1975 • 110 citations

A study on the influence of the concentration of Mg2+, Pi, K+, Na+, and tris on (Mg2+ + Pi)-supported g-strophanthin binding to (Na+ + K+)-activated ATPase from ox brain

1973 • 106 citations

Molecular weight of (sodium(1+), potassium(1+) ion)-stimulated ATPase from shark rectal gland

1979 • 105 citations

Solubilization and molecular weight determination of the from rectal glands of Squalus acanthias

1979 • 96 citations

Sodium- and potassium-activated adenosine triphosphatase of the nasal salt gland of the duck (Anas platyrhynchos). Purification, characterization, and NH2-terminal amino acid sequence of the phosphorylating polypeptide.

1976 • 88 citations

Studies on the characterization of the sodium—Potassium transport adenosine triphosphatase

1974 • 86 citations

Reaction of (sodium(1+)-potassium(1+) ion)-activated ATPase with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole: evidence for an essential tyrosine at the active site

1978 • 85 citations

Large scale partial purification and molecular and kinetic properties of the (Na + K)-activated adenosine triphosphatase from Artemia salina nauplii

1978 • 84 citations

The interaction of ATP‐analogues possessing a blocked gamma‐phosphate group with the sodium pump in human red cells.

1975 • 81 citations

On the specificity of the ATP-binding site of (Na+ + K+)-activated ATPase from brain microsomes

1971 • 78 citations

Elementary steps of the (Na+ + K+)-ATPase mechanism, studied with formycin nucleotides

1978 • 73 citations

An essential arginine residue in the ATP-binding centre of (Na+ + K+)-ATPase

1977 • 72 citations

Activation of beef heart mitochondrial adenosine triphosphatase by 2,4-dinitrophenol

1973 • 71 citations

The Titration of the Cardiac Glycoside Binding Site of the (Na+ + K+)-Adenosine Triphosphatase

1972 • 66 citations

The equilibrium between different conformations of the unphosphorylated sodium pump: effects of ATP and of potassium ions, and their relevance to potassium transport

1980 • 63 citations

Evidence that ouabain binds to the same large polypeptide chain of dimeric (sodium(1+), potassium(1+) ion)-stimulated ATPase that is phosphorylated from Pi

1979 • 58 citations

Mutual Exclusion of ATP, ADP and g-Strophanthin Binding to NaK-ATPase

1971 • 56 citations

Fluorescence properties of 2′ (or 3′)-O-(2,4,6-trinitrophenyl) adenosine 5′-triphosphate and its use in the study of binding to heavy meromyosin ATPase

1976 • 51 citations

Binding of ligands by proteins: a simple method with sephadex gel

1971 • 51 citations

A simple procedure for the preparation of highly purified (sodium + potassium) adenosinetriphosphatase from the rectal salt gland of Squalus acanthias and the electric organ of Electrophorus electrieus

1978 • 40 citations

The (Na+ + K+)-dependent ATPase: mode of inhibition of ADP/ATP exchange activity by MgCl2

1976 • 38 citations

EXPERIMENTAL AND THEORETICAL EXAMINATION OF THE FLIP‐FLOP MODEL OF (N<scp>a</scp>, K)‐ATP<scp>ase</scp> FUNCTION

1974 • 38 citations

Photoaffinity labelling of a small protein component of a purified (Na⁺‐K⁺)ATPase

1979 • 36 citations

ADP binding to (Na+ + K+)-activated ATPase

1973 • 35 citations

Fluorescence studies of the sodium and potassium transport adenosine triphosphatase labeled with fluorescein mercuric acetate and anthroylouabain.

1980 • 34 citations

Molecular weight of Na, K-ATPase approximated by the radiation inactivation method

1967 • 33 citations

Mathematical modelling of ATP, K+ and Na+ interactions with (Na+ + K+)-ATPase occurring under equilibrium conditions

1979 • 26 citations

Kinetics of cardiac glycoside binding to sodium, potassium adenosine triphosphatase studied with a fluorescent derivative of ouabain

1980 • 24 citations

Energy transformation in biological systems

1976 • 20 citations

Characterization of essential arginyl residues in sheep kidney (Na+ + K+)-ATPase

1979 • 18 citations

Fluorescence properties of a Meisenheimer complex

1974 • 16 citations