Evidence that an ATPase functions in the maintenance of the acidic pH of the hamster sperm acrosome.

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1984 • 4,899 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

Yeast/E. coli shuttle vectors with multiple unique restriction sites

1986 • 1,399 citations

Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: Results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes

1980 • 1,256 citations

A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector

1987 • 1,181 citations

Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria.

1982 • 1,152 citations

Biogenesis of Peroxisomes

1985 • 1,108 citations

Protein kinase recognition sequence motifs

1990 • 1,014 citations

Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experiments

1979 • 967 citations

Filter replicas and permanent collections of recombinant DNA plasmids

1979 • 355 citations

Catabolite inactivation in yeast

1976 • 332 citations

Peroxisomal protein import is conserved between yeast, plants, insects and mammals.

1990 • 296 citations

Glycolytic enzymes of Trypanosoma brucei. Simultaneous purification, intraglycosomal concentrations and physical properties

1986 • 216 citations

Targeted deletion of a yeast enolase structural gene. Identification and isolation of yeast enolase isozymes.

1982 • 166 citations

Evolutionary conservation of a microbody targeting signal that targets proteins to peroxisomes, glyoxysomes, and glycosomes.

1991 • 166 citations

NAD(+)-dependent isocitrate dehydrogenase. Cloning, nucleotide sequence, and disruption of the IDH2 gene from Saccharomyces cerevisiae.

1991 • 136 citations

Association of glyoxylate and beta-oxidation enzymes with peroxisomes of Saccharomyces cerevisiae

1990 • 136 citations

The presence of a histidine-aspartic acid pair in the active site of 2-hydroxyacid dehydrogenases. X-ray refinement of cytoplasmic malate dehydrogenase.

1983 • 135 citations

Glyoxysomal malate dehydrogenase from watermelon is synthesized with an amino-terminal transit peptide.

1990 • 115 citations

Subunit structure, expression, and function of NAD(H)-specific isocitrate dehydrogenase in Saccharomyces cerevisiae

1990 • 102 citations

Cloning and characterization of the gene encoding the IDH1 subunit of NAD(+)-dependent isocitrate dehydrogenase from Saccharomyces cerevisiae.

1992 • 101 citations

Isolation and expression of the gene encoding yeast mitochondrial malate dehydrogenase

1987 • 101 citations

Nucleotide sequence of the malate dehydrogenase gene of Thermus flavus and its mutation directing an increase in enzyme activity.

1986 • 100 citations

Isolation, nucleotide sequence, and disruption of the Saccharomyces cerevisiae gene encoding mitochondrial NADP(H)-specific isocitrate dehydrogenase

1991 • 89 citations

The three-dimensional structure of porcine heart mitochondrial malate dehydrogenase at 3.0-A resolution.

1986 • 79 citations

Cloning and sequence analysis of cDNAs encoding mammalian cytosolic malate dehydrogenase. Comparison of the amino acid sequences of mammalian and bacterial malate dehydrogenase.

1987 • 70 citations

The Malate Dehydrogenase Isoenzymes of Saccharomyces cerevisiae. Purification, Characterisation and Studies on Their Regulation

1978 • 60 citations

Gene sequence and primary structure of mitochondrial malate dehydrogenase from Saccharomyces cerevisiae

1988 • 56 citations

Complete nucleotide sequence of theEscherichia coligene encoding malate dehydrogenase

1987 • 50 citations

Glucose-induced degradation of the MDH2 isozyme of malate dehydrogenase in yeast.

1992 • 50 citations

Isoenzymes of Malate Dehydrogenase in Saccharomyces cerevisiae

1968 • 45 citations

Cloning and sequence analysis of cDNAs encoding mammalian mitochondrial malate dehydrogenase

1987 • 40 citations

Development of multipurpose peroxisomes in Candida boidinii grown in oleic acid-methanol limited continuous cultures

1992 • 37 citations

Structural and functional effects of mutations altering the subunit interface of mitochondrial malate dehydrogenase

1991 • 20 citations

Studies on yeast peroxisomal citrate synthase

1991 • 18 citations

Action of surfactants on porcine heart malate dehydrogenase isoenzymes and a simple method for the differential assay of these isoenzymes

1986 • 7 citations